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Free Estrone Saliva ELISA Kit

  • Created on the 22 February, 2017.

BACKGROUND

Estrone which is also known as 3-hydroxy-1,3,5 (10)-estratrien–17-one, is primarily produced by androstenedione and it is one of the three naturally occurring estrogens besides estradiol and estriol. The physiological role of endogenous estrone has not been well defined presently, however, many bioassay data are indicating that the estrogenic activity of estrone is considerably lower when compared to that of estradiol. Some of the characteristic of estrone indicate an essential function during the developing female sex organs and the secondary sex characteristics.

In women, men, postmenopausal women and prepubertal children the major portion of estrone is found to be derived from conversion of peripheral tissues. Also, premenopausal women are able to secrete more than 50% estrone through the ovaries. Following menopause, there is no dramatic decline in the concentrations of estrone levels when compared to that experienced for estradiol levels. In males the levels of E1 have been reported to increase inversely with age to that of 17-OH-progesterone. In post-menopausal women estrone represents that major form of estrogen, also there is evidence that excessive estrone levels can result from large amounts of androstenedione being converted in patients with ovarian tumors and polycystic ovary syndrome.

The present salivary free estrone ELISA assay is designed to provide a stress free procedure which is also non-invasive in order to generate comparable results to other sample and methods.

INTENDED USE

Human free estrone in saliva ELISA kit is an accurate in vitro quantitative method for analysing free estrone in human saliva samples. This assay has a minimum sensitivity detection limit of 4.7 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of free estrone using this human free estrone in saliva ELISA kit was approximately 4.7 pg/ml. The dynamic assay range for this kit is 10.0 – 300.0 pg/ml.

REFERENCES

  1. Increasing saliva (free) oestriol to progesterone ratio in late pregnancy: a role for oestriol in initiating spontaneous labour in man? Br Med J (Clin Res Ed). (1984) 289 (6443): 457-9. McGarrigle H.H. and Lachelin G.C.
  2. Saliva oestriol, oestradiol, oestrone and progesterone levels in pregnancy: spontaneous labour at term is preceded by a rise in the saliva oestriol:progesterone ratio. Br J Obstet Gynaecol. (1987) 94 (3): 227-35. Darne J., et al.
  3. Solid-phase enzymoimmunoassay of estrone in saliva. Clin Chem. (1989) 35 (4): 569-72. Folan J., et al.
  4. Metabolism of estrone and progesterone in vitro in human saliva. J Steroid Biochem. (1975) 6 (10): 1455-8. Elattar T.M.

ADDITIONAL INFORMATION

  • Full Name: Free Estrone Saliva ELISA Kit
  • Reactivity: Human
  • Sample Type: Saliva
  • Sensitivity: 4.7 pg/ml

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