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Free Estriol Saliva ELISA Kit

  • Created on the 22 February, 2017.

BACKGROUND

Estriol (also known as oestriol and E3) is significantly produced in large amounts during pregnancy due to the fetus. It is regarded as of the one of the three main estrogens that are made by the human body, where its production is dependent on an intact maternal-placental-fetal unit. Due to this fact, the measurement of maternal estriol concentration has been particularly useful in monitoring the fetal status during pregnancy especially in the third trimester. DHEA-S is produced by the adrenal cortex of the fetus and this can be converted to estriol by the placenta. Fetal placental is responsible for producing estriol which results in a progressive increase in the maternal circulating levels that can reach a late gestational peak of many orders of magnitude greater than in non-pregnant levels. If estriol concentrations are abnormally low in a pregnant woman, this can be an indication of a problem with the development of the child.

Many different bodily fluids can be routinely detected for estriol (E3) in order to monitor and manage the fetal well-being. There are also many advantages for measuring free-estriol instead of total serum or urinary E3. Free estriol is found to reflect more accurately the fetal outcome in diabetic pregnancies, since the levels are not affected by certain antibiotics administration or certain maternal hepatic and renal related diseases. Also, the fact that frequent measurement of free-estriol is highly recommended since the ranges for normal and abnormal levels are relatively wide and overlapping. The current salivary ELISA assay is intended to measure free estriol to provide acceptable results that are comparable to those using serum samples. It offers the advantage of being a non-invasive process which does not put the patient under any unnecessary stress.

INTENDED USE

Human free estriol in saliva ELISA kit is an accurate in vitro quantitative method for detecting free estriol in human saliva samples. This assay has a minimum sensitivity detection limit of 1.4 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of estriol (unconjugated estriol) using this human free estriol in saliva ELISA kit was approximately 1.4 pg/ml. The dynamic assay range for this kit is 2.5 – 4,000.0 pg/ml.

REFERENCES

  1. Enzymeimmunoassay of unconjugated estriol in serum and saliva during pregnancy. Exp Clin Endocrinol. (1985) 86 (2): 178-84. Weissbach A., et al.
  2. ELISA for salivary and plasma estriol in pregnancy. Steroids. (1984) 43 (5): 469-79. Preti M.S., et al.
  3. The measurement of hormones in saliva: possibilities and pitfalls. J Steroid Biochem. (1987) 27 (1-3): 81-94. Review. Vining R.F. and McGinley R.A.
  4. Salivary unconjugated estriol levels in normal third trimester pregnancy – direct correlation with serum levels. Steroids. (1983) 41 (2): 145-53. Kundu N., et al.

ADDITIONAL INFORMATION

  • Full Name: Free Estriol Saliva ELISA Kit
  • Reactivity: Human
  • Sample Type: Saliva
  • Sensitivity: 1.4 pg/ml

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