Free DHEA Saliva ELISA Kit (Dehydroepiandrosterone)

INTENDED USE

Human free DHEA saliva ELISA kit is an accurate in vitro quantitative method for detecting free dehydroepiandrosterone (DHEA) in human saliva samples. This assay has a minimum sensitivity detection limit of 3.7 pg/ml.

BACKGROUND

Dehydroepiandrosterone (DHEA) which is also known as androstenolone or 3b-hydroxy-5-androsten-17-one, is a member of the androgens family and it is produced in the adrenal cortex. There are also small amounts which can be produced in the brain or the gonads. The normal concentration changes significantly with age and this age-related decline is known as “adrenopause”, this is primarily caused by a reduction in the production within the adrenal cortex. Its measurement is helpful due to its ubiquitous properties in many fields of research and diagnostics (for example endocrinology, neuroendocrinology, anti-aging medicine, veterinary medicine, in sports medicine and psychology).

It is only the non-bound or free DHEA which has the ability to enter cells and then be converted, this is also the case with saliva. This has made it easier to determine the biologically active concentrations by directly and easily measuring saliva levels instead of blood, since in saliva only the free active form is present. However, one disadvantage of saliva sampling over blood is that the concentration of free DHEA in saliva is approx. 1/30 of that found in sera. Both in serum and also in salivary the concentrations that are observed can be seen to pulsatile with clear oscillation range around the middle value and because of the dynamic pulsatile nature of the pattern of secretion, it is highly recommended to use saliva sampling in order to make repeated measurements.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiterwells (96 Wells): Coated with polyclonal anti-DHEA antibody.
• Calibrator 0.
• Calibrators 1-5: Concentrations 10, 40, 160, 640, 2560 pg/ml.
• Control 1 (Low) And Control 2 (High).
• Enzyme Conjugate: Consisting of antibody conjugated to horseradish peroxidase (HRP).
• TMB Substrate Solution.
• Stop Solution.
• Wash Solution (10x Concentrated).

SENSITIVITY

The minimum sensitivity detection limit of free dehydroepiandrosterone (DHEA) using current DHEA saliva ELISA kit was approximately 3.7 pg/ml. The dynamic range for this assay is 10.0 – 2560.0 pg/ml.

ASSAY CHARACTERISTICS

– Analytical Sensitivity: 3.7 pg/ml
– Assay Dynamic Range: 10 – 2560 pg/ml.
– Intra-Assay Variation: 4.9 – 9.0%
– Inter-Assay Variation: 4.5 – 6.8%
– Recovery: 96 – 112%
– Linearity: 72 – 108%

REFERENCES

1. Dehydroepiandrosterone sulfate in saliva. Acta Obstet Gynecol Scand. (1988) 67 (4): 359-61. Lagrelius A., et al.
2. The measurement of hormones in saliva: possibilities and pitfalls. J Steroid Biochem. (1987) 27 (1-3): 81-94. Review. Vining R.F. and McGinley R.A.
3. Correlations between serum and salivary hormonal concentrations in response to resistance exercise. J Sports Sci. (2008) 26 (10): 1067-72. Cadore E., et al.

ADDITIONAL INFORMATION

• Full Name: Free DHEA Saliva ELISA Kit (Dehydroepiandrosterone)
• Reactivity: Human
• Sample Type: Saliva
• Sensitivity: 3.7 pg/ml

OTHER RELATED ELISA KITS

• Cortisol Saliva ELISA Kit
• Androstenedione Saliva ELISA Kit
• Estrone Saliva ELISA Kit