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Free Beta-Subunit Human Chorionic Gonadotropin (Free Beta-hCG) ELISA Kit

  • Created on the 15 February, 2017.

BACKGROUND

Free beta chorionic gonadotropin (Free beta hCG) is a glycoprotein hormone that is produced by the placenta during pregnancy. Structurally intact hCG molecules are made up of two polypeptide subunits which are non-covalently linked together – alpha and beta chain subunits. The measurement of alpha subunit of hCG is known to give similar results whereas measuring the beta subunits doesn’t. Therefore monoclonal antibodies which are able to recognise unique sites on the beta chain of the ß-hCG/hCG molecule play an essential role in differentiating between hCG and FSH, LH and TSH hormones.

The development of specific assay makes it possible for early detection of beta-hCG during pregnancy and this has been vital in screening for Down syndrome and trisomy 18. Pregnancies tend to have higher levels of HCG compared to other others where the fetus has Down syndrome and where the fetus has trisomy 18 then the levels of HCG are much lower. There are also evidence that high levels of ß-hCG/hCG may be associated with chorionepithelioma, seminoma, embryonal cell carcinoma, neoplasms of trophoblastic and nontrophoblastic origin (eg. hydatiform mole).

INTENDED USE

Free beta-hCG ELISA kit is a reliable quantitative procedure for measuring free beta-subunit of human chorionic gonadotropin (free beta-hCG) in serum. This assay has minimum sensitivity detection limit of 0.25 ng/ml.

SENSITIVITY

The minimum sensitivity detection limit of free beta-subunit chorionic gonadotropin (free beta-hCG) using this free beta-hCG ELISA kit was approximately 0.25 ng/ml. The dynamic assay range for this kit is 0.25 – 50.0 ng/ml.

REFERENCES

  1. The biological and clinical significance of nicks in human chorionic gonadotropin and its free-beta-subunit. Yale J Biol Med. (1991) 64 (6): 627-37. Review. Cole L.A., et al.
  2. Physiological studies of human chorionic gonadotropin (hCG), αhCG, and βhCG as measured by specific monoclonal immunoradiometric assays. Endocrinology (1987) 120: 549-558. Ozturk, M., et al.
  3. The stability of blood samples for the measurement of the free β subunit of chorionic gonadotrophin. Prenat. Diagn. (1995) 15: 94-95. Densem J., and Wald N.J.
  4. Serum concentration of human chorionic gonadotropin and its alpha and beta subunits. Clin. Endocrinol. (OXF), (1980) 13: 319-329. Gaspard U.J., et al.
  5. The beta-core fragment of human chorionic gonadotropin: biochemical background and clinical application. Anticancer Res. (1997) 17 (2B): 1255-72. Review. Schutter E.M., et al.

ADDITIONAL INFORMATION

  • Full Name: Free Beta Subunit Human Chorionic Gonadotropin (Free Beta-hCG) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 0.25 ng/ml

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