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FPV (Feline Panleukopenia Virus) ELISA Kit (Feline)

INTENDED USE

FPV ELISA kit is intended for analysing antibodies against feline panleukopenia virus (FPV) in samples of serum or plasma. This assay has a quantitative and qualitative standard range.

BACKGROUND

Feline panleukopenia virus (FPV), is a life threatening and highly contagious viral disease found in cats. It is primarily responsible for affecting the rapid dividing blood cells which can be found in the intestinal tract, bone marrow and stem cells (developing fetus). There is evidence to indicate the FPV is also able to mimic many other diseased conditions such as feline immunodeficiency virus (FIV), feline leukemia (FeLV), pancreatitis and poisoning. For diagnosis of FPV infection and vaccination control, measuring antibody titre is one of the most common procedure used. This involves having a virus being attached to the solid phase via monoclonal antibodies that are able to catch the antibodies which have been induced as a result of the either infection or vaccination. Antibody titers which are greater than 1:1350 dilutions are then considered to be protected. Other test are available to analyse the virus in faeces, but the isolation step can be quite time consuming and the test itself is expensive. Also, any recent vaccinations against panleukopenia will result in producing a false positive.

FELINE PANLEUKOPENIA VIRUS ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Strips: Coated with monoclonal FPV antibodies.
  • Strip Holder.
  • Inactivated Feline Panleukopenia Virus Antigen.
  • ELISA Buffer.
  • HRPO Conjugate Anti-Species Antibodies
  • Positive Control.
  • Negative Control.
  • Substrate A.
  • Substrate B.
  • Wash Solution (200x Concentrated).
  • Stop Solution.
  • Plastic Cover Seal.

SENSITIVITY

The standard range of feline panleukopenia virus (FPV) using current FPV ELISA Kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Feline Panleukopenia Virus (FPV): ELISA
– Positive control should give an OD (450nm) > 1,000.
– Negative control must give an OD (450nm) lower than 0,300.
– A sample is considered positive when the measured extinction is 0.150 higher than the OD of the negative control.

REFERENCES

  1. Detection of protective antibody titers against feline panleukopenia virus, feline herpesvirus-1, and feline calicivirus in shelter cats using a point-of-care ELISA. J Feline Med Surg. (2011) 13 (12): 912-8. Digangi B.A., et al.
  2. Evaluation of an in-house dot enzyme-linked immunosorbent assay to detect antibodies against feline panleukopenia virus. J Feline Med Surg. (2014) 16 (10): 805-11. Mende K., et al.
  3. Feline parvovirus infection and associated diseases. Vet J. (2014) 201 (2): 150-5. Review. Stuetzer B. and Hartmann K.
  4. Detection of feline panleukopenia virus using a commercial ELISA for canine parvovirus. Vet Ther. (2009) 10 (4): E1-6. Abd-Eldaim M., et al.
  5. Vaccination against Feline Panleukopenia: implications from a field study in kittens. BMC Vet Res. (2012) 8: 62. Jakel V., et al.

ADDITIONAL INFORMATION

  • Full Name: FPV (Feline Panleukopenia Virus) ELISA Kit (Feline)
  • Reactivity: Feline
  • Sample Type: Serum, Plasma
  • Sensitivity: Qualitative, Quantitative

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