- Created on the 15 February, 2017.
Human FSH ELISA kit is a reliable quantitative procedure for measuring follicle-stimulating hormone (FSH) in human serum samples. This assay has a minimum sensitivity detection limit of 0.856 mIU/ml.
Follicle-stimulating hormone (FSH) is a glycoprotein which is known to be secreted by basophil cells that are located in the anterior pituitary. The release of FSH from the anterior pituitary is regulated by gonadotropin-releasing hormone (GnRH) which is produced by the hypothalamus. FSH is just like to other glycoproteins being made up of an alpha and beta subunit. The immunological and biological properties of these glycoproteins is determined solely by the uniqueness of the beta subunits whilst the alpha subunits remain very similar structurally.
Alongside luteinizing hormone (LH), FSH is able to regulate the synthesis and secretion of female and male sex hormones via a negative feedback relationship, together they are able to control the growth and reproductive activities of gonadal tissues. Elevated concentrations of FSH have been identified after menopause, premature ovarian failure and after castration. Due to FSH being regulated by a negative feedback mechanism, the concentrations of FSH can be normalized by administration of estrogens. Any abnormalities between the relationship of LH and FSH or estrogen and FSH have been demonstrated to be associated with anorexia nervosa and polycystic ovarian disease. There are exception with ovarian failure when random FSH levels exceed 40 mIU/mL.
Reagents which are supplied with the FSH ELISA kit, please following instructions for storage conditions.
1. Microtiter Plate: monoclonal anti-FSH antibody coated.
2. Standard (Standard 0-5): 0; 5; 10; 20; 50; 100 mIU/mL.
3. Enzyme Conjugate.
4. Substrate Solution.
5. Stop Solution.
Note: Additional Standard 0 for sample dilution is available on request.
The minimum sensitivity detection limit of follicle-stimulating hormone using current human FSH ELISA kit was approximately 0.856 mIU/ml. The dynamic range for this assay is 5.0 – 100.0 mIU/ml.
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- Follicle stimulating hormone and its receptor: future perspectives. Hum Reprod. (1998) 13 Suppl 3: 18-35. Review. Chappel S., et al.
- The importance of consistent FSH delivery in infertility treatment. Reprod Biomed Online. (2006) 12 (4): 493-9. Review. Levi Setti P.E.
- Full Name: Follicle-Stimulating Hormone (FSH) ELISA Kit
- Reactivity: Human
- Sample Type: Serum
- Sensitivity: 0.856 mIU/ml