- Created on the 10 October, 2017.
Folic acid (known as folate and vitamin B9) is one of the main B vitamins, it plays a vital role in making the genetic material of cells and is also essential for the growth and development processes. Other important roles include helping to maintain a healthy nervous system and forming of red blood cells which are needed to transport oxygen around the body. These wide range of functions makes folic acid a key vitamin for cell growth and metabolism. Folic acid supplements are highly recommended for pregnant women, since they lower the risk of early labour and pre-eclampsia. Supplements have also been used to treat many different types of anaemia, folate deficiencies are common in people having kidney or liver disease, digestive problems and individuals who abuse alcohol. Folic acid is found to reduce the toxicity of methotrexate when used in treating rheumatoid arthritis and psoriasis. Some of the traditional methods used for analysis include microbiological, HPLC and TLC, however, these involve complex equipment and are relatively time consuming. The current ELISA assay offers a quick alternative to measure folic acid in supplemented foods. Providing a result in 2-4 hours when compared to 24-48 hours using some of the traditional techniques.
Folic acid ELISA kit can be used to determine quantitative concentrations of folic acids in food. This assay has a minimum analytical sensitivity limit of 2.0 ng/ml.
The minimum detection sensitivity level of folic acid (vitamin B9 and folate) using this folic acid ELISA kit was 2.0 ng/ml. The standard assay range for this kit is 4.0 – 400.0 ng/ml.
- Disposable microfluidic ELISA for the rapid determination of folic acid content in food products. Anal Bioanal Chem. (2007) 387 (1): 267-75. Hoegger D., et al.
- Determination of folate/folic acid level in milk by microbiological assay, immuno assayand high performance liquid chromatography. J Dairy Res. (2013) 80 (2): 233-9. Iyer R. and Tomar S.K.
- Addition of cobalamin to iron and folic acid improves hemoglobin rise in nutritional anemia. Indian J Pediatr. (2012) 79 (12): 1592-6. Chandelia S., et al.
- Antibody to folic acid: increased specificity and sensitivity in ELISA by using epsilon-aminocaproic acid modified BSA as the carrier protein. J Immunol Methods. (1995) 184 (1): 1-6. Das Sarma J., et al.
- Full Name: Folic Acid ELISA Kit
- Sample Type: Food
- Sensitivity: 2.0 ng/ml