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Folic Acid ELISA Kit

  • Created on the 10 October, 2017.

BACKGROUND

Folic acid (known as folate and vitamin B9) is one of the main B vitamins, it plays a vital role in making the genetic material of cells and is also essential for the growth and development processes. Other important roles include helping to maintain a healthy nervous system and forming of red blood cells which are needed to transport oxygen around the body. These wide range of functions makes folic acid a key vitamin for cell growth and metabolism. Folic acid supplements are highly recommended for pregnant women, since they lower the risk of early labour and pre-eclampsia. Supplements have also been used to treat many different types of anaemia, folate deficiencies are common in people having kidney or liver disease, digestive problems and individuals who abuse alcohol. Folic acid is found to reduce the toxicity of methotrexate when used in treating rheumatoid arthritis and psoriasis. Some of the traditional methods used for analysis include microbiological, HPLC and TLC, however, these involve complex equipment and are relatively time consuming. The current ELISA assay offers a quick alternative to measure folic acid in supplemented foods. Providing a result in 2-4 hours when compared to 24-48 hours using some of the traditional techniques.

INTENDED USE

Folic acid ELISA kit can be used to determine quantitative concentrations of folic acids in food. This assay has a minimum analytical sensitivity limit of 2.0 ng/ml.

SENSITIVITY

The minimum detection sensitivity level of folic acid (vitamin B9 and folate) using this folic acid ELISA kit was 2.0 ng/ml. The standard assay range for this kit is 4.0 – 400.0 ng/ml.

REFERENCES

  1. Disposable microfluidic ELISA for the rapid determination of folic acid content in food products. Anal Bioanal Chem. (2007) 387 (1): 267-75. Hoegger D., et al.
  2. Determination of folate/folic acid level in milk by microbiological assay, immuno assayand high performance liquid chromatography. J Dairy Res. (2013) 80 (2): 233-9. Iyer R. and Tomar S.K.
  3. Addition of cobalamin to iron and folic acid improves hemoglobin rise in nutritional anemia. Indian J Pediatr. (2012) 79 (12): 1592-6. Chandelia S., et al.
  4. Antibody to folic acid: increased specificity and sensitivity in ELISA by using epsilon-aminocaproic acid modified BSA as the carrier protein. J Immunol Methods. (1995) 184 (1): 1-6. Das Sarma J., et al.

ADDITIONAL INFORMATION

  • Full Name: Folic Acid ELISA Kit
  • Sample Type: Food
  • Sensitivity: 2.0 ng/ml

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L. Johnston
PhD Student / University of Glasgow

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Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford