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Fish (Parvalbumin) ELISA Kit

  • Created on the 6 October, 2017.

BACKGROUND

Fish has been a vital source of dietary protein and in many places it represent the major component of the diet, especially near coastal areas. However, allergy to fish is a prevalent cause for a large number of food allergic reactions. One of the major allergen in fish is the parvalbulin, this a 12 kDa protein and it is about 108 amino acids in length. The abundant fish parvalbumin was one of the first food allergens to be isolated, crystallised and then identified on a molecular level. Parvalbumin are sarcoplasmatic muscle proteins which are highly water-soluble, well conserved and are resistant to extreme pH, denaturing agents and heat. One of the major function of this protein is in calcium buffering and this is thought to play a role in muscle relaxation. This ELISA assay offers a highly sensitive detection method for fish that is based on the parvalbumin allergen. It can be used to quantify fish residues present in soups, wine, crackers, surimi, sauces and many products used in the Asian culture.

INTENDED USE

Fish ELISA kit is designed for detecting quantitative levels of fish (in particular allergen parvalbumin) in many different foods (such as sauces, soups, crackers, wine, Asian food and surimi). This assay has a minimum analytical sensitivity limit of 1.4 ppm.

SENSITIVITY

The minimum detection sensitivity level of fish (parvalbumin allergen) using this fish ELISA kit was 1.4 ppm. The standard assay range for this kit is 4.0 – 100.0 ppm.

REFERENCES

  1. Immunological cross-reactivity between four distant parvalbumins-Impact on allergen detection and diagnostics. Mol Immunol. (2015) 63 (2): 437-48. Sharp M.F., et al.
  2. A sensitive enzyme-linked immunosorbent assayfor the determination of fish protein in processed foods. Food Chem. (2013) 136 (2): 675-81. Shibahara Y., et al.
  3. Monoclonal antibody specific to a major fish allergen: parvalbumin. J Food Prot. (2009) 72 (4): 818-25. Gajewski K.G. and Hsieh Y.H.
  4. Quantitative sandwich ELISA for the determination of fish in foods. J Immunol Methods. (2008) 329 (1-2): 45-55. Faeste C.K. and Plassen C.
  5. Protein carbonylation during electron beam irradiation may be responsible for changes in IgE binding to turbot parvalbumin. Food Chem Toxicol. (2014) 69: 32-7. Li Z., et al.
  6. Parvalbumin–the major tropical fish allergen. Pediatr Allergy Immunol. (2008) 19 (5): 399-407. Lim D.L., et al.

ADDITIONAL INFORMATION

  • Full Name: Fish (Parvalbumin) ELISA Kit
  • Sample Type: Food
  • Sensitivity: 1.4 ppm

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