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FGF9 ELISA Kit

INTENDED USE

Human FGF9 ELISA kit is a reliable quantitative procedure for measuring fibroblast growth factor-9 (FGF-9, FGF9) in human serum, plasma, biological fluids and tissue homogenates. This assay has a minimum sensitivity detection limit of 37.5 pg/ml.

BACKGROUND

Human FGF9 (fibroblast growth factor-9, FGF-9) is a steroid-regulated mitogen and possesses a broad range of cell survival and mitogenic activities that can be involved in a number of different biological processes such as cell growth, morphogenesis, tissue repair, invasion, embryonic development and tumor growth. Human FGF9 cDNA has been cloned using oligonucleotide probes that encode a polypeptide consisting of 208 amino acids and it share approx. 30% sequence similarity to the other FGF family members. The standard that is used in the following FGF-9 ELISA kit is a recombinant anti human FGF9 that is made up of 208 amino acids and has a molecular mass of 23KDa, due to glycosylation, the molecular mass can be between 25-27KDa.

Fibroblast growth factor-9 is produced and secreted by the prostatic stromal cells, it is a survival factor for mesenchymal or nerve cells and a potent mitogen activator for both prostatic stromal or epithelial cells in culture. FGF9 is a growth factor that is abundantly secreted and can act as either an autocrine mitogen for stromal cells or a paracrine mitogen for epithelial cells. There is evidence that overexpression of this growth factor can play a vital role in epithelial or stromal proliferation in benign prostatic hyperplasia.

FGF9 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti FGF-9 antibody.
  • Standards: Concentrations 0, 62.5, 125, 250, 500, 1000, 2000, 4000 pg/ml, made up from lyophilized recombinant human fibroblast growth factor-9 (FGF9).
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled FGF-9 Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum sensitivity detection limit of fibroblast growth factor-9 (FGF-9, FGF9) using current human FGF9 ELISA kit was approximately 37.5 pg/ml. The dynamic range for this assay is 62.5 – 4,000.0 pg/ml.

ASSAY CHARACTERISTICS

– Fibroblast Growth Factor-9 (FGF9): ELISA
– Specificity: Highly specific for FGF9, no cross reactivity or interference between FGF-9 and analogues was detected.
– Recovery: Serum (87 – 103%), EDTA Plasma (85 – 104%), Heparin Plasma (87 – 100%).
– Linearity: Serum (85 – 105%), EDTA Plasma (82 – 100%), Heparin Plasma (80 – 99%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. FGF9 on the move. Nat Genet. (2009) 41 (3): 272-3. Spicer D.
  2. Accumulation of FGF-9 in prostate cancer correlates with epithelial-to-mesenchymal transition and induction of VEGF-A expression. Anticancer Res. (2014) 34 (2): 695-700. Teishima J., et al.
  3. Autoinhibitory mechanism for the mutation-induced impaired FGF9 signaling. J Chem Inf Model. (2012) 52 (9): 2422-9. Wang Y., et al.
  4. FGF-9 is an autocrine and paracrine prostatic growth factor expressed by prostatic stromal cells. J Cell Physiol. (1999) 180 (1): 53-60. Giri D., Ropiquet F. and Ittmann M.

ADDITIONAL INFORMATION

  • Full Name: FGF9 ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Biological Fluids, Serum, Tissue Homogenates
  • Sensitivity: 37.5 pg/ml

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