GET A QUOTE

FGF9 ELISA Kit

INTENDED USE

Human FGF9 ELISA kit is a reliable quantitative procedure for measuring fibroblast growth factor-9 (FGF-9, FGF9) in human serum, plasma, body fluids, cell culture supernatant or tissue lysates. This assay has a minimum sensitivity detection limit of < 15.0 pg/ml.

BACKGROUND

Human FGF9 (fibroblast growth factor-9, FGF-9) is a steroid-regulated mitogen and possesses a broad range of cell survival and mitogenic activities that can be involved in a number of different biological processes such as cell growth, morphogenesis, tissue repair, invasion, embryonic development and tumor growth. Human FGF9 cDNA has been cloned using oligonucleotide probes that encode a polypeptide consisting of 208 amino acids and it share approx. 30% sequence similarity to the other FGF family members. The standard that is used in the following human FGF9 ELISA kit is a recombinant anti human FGF9 that is made up of 208 amino acids and has a molecular mass of 23KDa, due to glycosylation, the molecular mass can be between 25-27KDa.

Human FGF9 is produced and secreted by the prostatic stromal cells, it is a survival factor for mesenchymal or nerve cells and a potent mitogen activator for both prostatic stromal or epithelial cells in culture. Human FGF9 is a growth factor that is abundantly secreted and can act as either an autocrine mitogen for stromal cells or a paracrine mitogen for epithelial cells. There is evidence that overexpression of this growth factor can play a vital role in epithelial or stromal proliferation in benign prostatic hyperplasia. This human FGF9 ELISA kit is suitable for analysing many different samples types for example; body fluids, serum, tissue lysates, cell culture supernatant and plasma.

FGF9 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Standards: Concentration 0-4000.0 pg/ml. Made up from lyophilized recombinant anti human FGF9.
  • One 96-well plate: Precoated with anti human FGF9 antigen.
  • Sample diluent buffer.
  • Biotinylated anti human FGF9 antibody.
  • Antibody diluent buffer.
  • Avidin biotin peroxidase complex (ABC).
  • ABC diluent buffer.
  • TMB colour developing agent.
  • TMB stop solution.

SENSITIVITY

The minimum sensitivity detection limit of fibroblast growth factor-9 (FGF-9, FGF9) using current human FGF9 ELISA kit was approximately < 15.0 pg/ml. The dynamic range for this assay is 62.5 – 4000.0 pg/ml.

REFERENCES

  1. FGF9 on the move. Nat Genet. (2009) 41 (3): 272-3. Spicer D.
  2. Accumulation of FGF9 in prostate cancer correlates with epithelial-to-mesenchymal transition and induction of VEGF-A expression. Anticancer Res. (2014) 34 (2): 695-700. Teishima J., et al.
  3. Autoinhibitory mechanism for the mutation-induced impaired FGF9 signaling. J Chem Inf Model. (2012) 52 (9): 2422-9. Wang Y., et al.
  4. FGF9 is an autocrine and paracrine prostatic growth factor expressed by prostatic stromal cells. J Cell Physiol. (1999) 180 (1): 53-60. Giri D., Ropiquet F. and Ittmann M.

ADDITIONAL INFORMATION

  • Full Name: FGF9 ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Body Fluids, Tissue Lysate, Cell Culture Supernatant
  • Sensitivity: < 15.0 pg/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford