FHV ELISA Kit (Feline Herpes Virus)

Full Name: FHV ELISA Kit (Feline Herpes Virus)
Reactivity: Feline
Sample Type: Serum, Plasma
Sensitivity: Qualitative

BACKGROUND

Feline herpes virus (also called FHV or FHV-1) is a contagious ubiquitous virus which is responsible for causing upper respiratory infections (URIs, also more commonly known as cat flu). In majority of instances, a specific diagnosis is usually not required and instead the typical signs are enough in order to make a presumptive diagnosis of FHV-infection. Cats which are affected by FHV show respiratory distress, sneezing and possibly excessive rubbing of the face on objects. It is possible for cats to progress from these initial symptoms to become ill and develop pneumonia or heart failure. This might happen if a sickness or injury causes the cat’s immune system to become compromised or undeveloped. Fever, fatigue, loss of appetite and coughing up blood are a few symptoms that could point to a more serious infection.

If there is a need for a specific diagnosis then this can be accomplished by various laboratory testing. These can include FHV isolation, serology (for example serum neutralizing titers and ELISA assays), ﬂuorescent antibody and PCR analysis. Biopsies may also reveal virus activity; this information is helpful for identifying skin infections brought on by FHV-associated dermatitis. The treatment process tends to be aimed at controlling the clinical signs and possibility reducing secondary complications.

INTENDED USE

FHV ELISA kit is designed for measuring antibodies against feline herpes virus (FHV or FHV-1) using plasma or serum samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Strips: Coated with monoclonal FHV antibodies.
Negative Control.
Positive Control.
FHV Antigen.
Concentrated HRPO Conjugate.
Conjugate Buffer.
Substrate A.
Substrate B.
Strip Holder.
ELISA Buffer.
Wash Solution (200x Concentrated).
Stop Solution.
Plastic Cover Seal.

SENSITIVITY

The standard range of feline herpes virus (FHV and FHV-1) using current FHV ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

REFERENCES

Detection of protective antibody titers against feline panleukopenia virus, feline herpes virus-1, and feline calicivirus in shelter cats using a point-of-care ELISA. J Feline Med Surg. (2011) 13 (12): 912-8. Digangi B.A., et al.
Enzyme-linked immunosorbent assay for detection of feline herpes virus 1 IgG in serum, aqueous humor, and cerebrospinal fluid. J Vet Diagn Invest. (1998) 10 (4): 315-9. Dawson D.A., et al.
Evaluation of subunit vaccines against feline immunodeficiency virus infection. Vaccine. (1996) 14 (4): 285-9. Verschoor E.J., et al.
Detection of feline herpes virus-specific antibodies and DNA in aqueous humor from cats with or without uveitis. Am J Vet Res. (1999) 60 (8): 932-6. Maggs D.J., et al.
Relative sensitivity of polymerase chain reaction assays used for detection of feline herpes virus type 1 DNA in clinical samples and commercial vaccines. Am J Vet Res. (2005) 66 (9): 1550-5. Maggs D.J. and Clarke H.E.

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