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Ferritin ELISA Kit

  • Created on the 12 February, 2017.

BACKGROUND

Ferritin is a water soluble, globular protein that can be mainly found in the liver and is responsible for iron storage. The molecule is composed of an apoferritin shell which comprises heavy and light subunits that are surrounded by a crystalline core that is made up of phosphate and iron oxide. Apoferritin has the ability to bind to free ferrous iron and then store this in a ferric state. Free iron is toxic to cells and the body has developed an elaborate set of protective mechanisms in order to bind iron in a number of different tissue compartments. Within the cells, iron can be found to be stored complexed to protein as either a ferritin or hemosiderin.

Ferritin is mainly synthesized in the liver, spleen and a number of other body tissues, with the major of the concentrations being found in the liver, bone marrow, spleen and intestinal mucosa. The levels of ferritin that are measured have a direct correlation to the total amount of iron stored within the body, for example; when ferritin levels are low this can indicate a risk of iron deficiency which may lead to anaemia, whereas when ferrin levels are elevated so is the levels of iron – in this case iron is usually excreted out of the body in the stool.

The measurement of serum ferritin can be carried out routinely and is a particularly useful indicator for the early detection of iron deficiency (anemia) in apparently healthy people. It is also regarded as clinically significant when monitoring the iron status for renal dialysis patients, blood donors and pregnant women. Early method that were utilised to detect iron levels were lacking in sensitivity and very invasive (causing unnecessary trauma to patients). Current immunoassay procedure to determine ferritin levels in serum offer better sensitivity and are non-invasive (very little patient discomfort).

INTENDED USE

Human ferritin ELISA kit is a reliable quantitative procedure for measuring ferrintin in human serum and plasma (heparin, EDTA) samples. This assay has a minimum sensitivity detection limit of 0.53 ng/ml.

SENSITIVITY

The minimum sensitivity detection limit of ferritin using this human ferritin ELISA kit was approximately 0.53 ng/ml. The dynamic assay range for this kit is 5.0 – 1000.0 ng/ml.

REFERENCES

  1. Ferritin as a Tumor Marker. J. Clin. Immunoassay (1983) 6/3: 234-240. Drysdale, J.W.
  2. Three Commercial Methods for Serum Ferritin Compared and the High-Dose “Hook Effect” Eliminated. Clin. Chem. (1983) 29/6: 1109-1113, 1983. Ronald, Ng. H., et al.
  3. Sensitive Sandwich Enzyme Immunoassay for Serum Ferritin on Microtitre Plates. Ann. Clin. Biochem. (1981) 18: 48-53. Sukanya, L., et al.
  4. Human ferritin for tumor detection and therapy. Wiley Interdiscip Rev Nanomed Nanobiotechnol. (2013) 5 (4): 287-98. Review. Fan K., Gao L. and Yan X.

ADDITIONAL INFORMATION

  • Full Name: Ferritin ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma (EDTA, Heparin)
  • Sensitivity: 0.53 ng/ml

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