Human ferritin ELISA kit is a reliable quantitative procedure for measuring ferritin in human serum and plasma (heparin, EDTA) samples. This assay has a minimum sensitivity limit of 0.04 ng/ml.
Ferritin is a water soluble, globular protein that can be mainly found in the liver and is responsible for iron storage. The molecule is composed of an apoferritin shell which comprises heavy and light subunits that are surrounded by a crystalline core that is made up of phosphate and iron oxide. Apoferritin has the ability to bind to free ferrous iron and then store this in a ferric state. Free iron is toxic to cells and the body has developed an elaborate set of protective mechanisms in order to bind iron in a number of different tissue compartments. Within the cells, iron can be found to be stored complexed to protein as either a ferritin or hemosiderin.
Ferritin is mainly synthesized in the liver, spleen and a number of other body tissues, with the major of the concentrations being found in the liver, bone marrow, spleen and intestinal mucosa. Also, there is a direct correlation to the total amount of iron stored within the body, for example; when ferritin levels are low this can indicate a risk of iron deficiency which may lead to anaemia, whereas when ferrin levels are elevated so is the levels of iron – in this case iron is usually excreted out of the body in the stool. The measurement of serum ferritin can be carried out routinely and is a particularly useful indicator for the early detection of iron deficiency (anemia) in apparently healthy people. It is also regarded as clinically significant when monitoring the iron status for renal dialysis patients, blood donors and pregnant women. Early method that were utilised to detect iron levels were lacking in sensitivity and very invasive (causing unnecessary trauma to patients). The current procedure to determine human ferritin levels in serum offer better sensitivity and are non-invasive (very little patient discomfort).
FERRITIN ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Zero Standard.
- Standard 1 – 5: Concentration 5 – 1000 ng/ml, made up from ferritin .
- Ferritin Control.
- Enzyme Conjugate: Consisting of human ferritin antibody conjugated with horseradish peroxidase (HRP).
- Microtiterwells: Wells coated with ferritin antigen.
- TMB Substrate Solution.
- Stop Solution: Sulphuric acid (avoid any skin contact).
- Wash Solution (10x Concentrated).
The minimum sensitivity detection limit of human ferritin using current ferritin ELISA kit was approximately 0.04 ng/ml. The dynamic range for this assay is 5.0 – 1000.0 ng/ml.
– Ferritin: ELISA
– Intra Assay Variation: Within ≤ 7.5%
– Inter Assay Variation: Within ≤ 6.1%
– Specificity: Liver Human Iso-Ferritin (100%), Spleen Human Iso-Ferritin (80%), Heart Human Iso-Ferritin (12%).
– Accuracy: Average value (±SD) of 98.66% ±2.90%, dilution test gave an average value (±SD) of 102.11% ± 5.32%.
– Sensitivity: 0.04 ng/mL at the 95 % confidence limit.
– Hook Effect: Shows no hook effect up to 50,000 ng/mL.
- Ferritin as a Tumor Marker. J. Clin. Immunoassay (1983) 6/3: 234-240. Drysdale, J.W.
- Three Commercial Methods for Serum Ferritin Compared and the High-Dose “Hook Effect” Eliminated. Clin. Chem. (1983) 29/6: 1109-1113, 1983. Ronald, Ng. H., et al.
- Human ferritin for tumor detection and therapy. Wiley Interdiscip Rev Nanomed Nanobiotechnol. (2013) 5 (4): 287-98. Review. Fan K., Gao L. and Yan X.
- Full Name: Ferritin ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma (EDTA, Heparin)
- Sensitivity: 0.04 ng/ml