Ferritin ELISA Kit
INTENDED USE
Human ferritin ELISA kit is a reliable quantitative procedure for measuring ferritin in human serum and plasma (heparin, EDTA) samples. This assay has a minimum sensitivity limit of 0.04 ng/ml.
BACKGROUND
Ferritin is a water soluble, globular protein found in the liver and it is responsible for iron storage. The molecule is composed of an apoferritin shell which comprises heavy and light subunits that are surrounded by a crystalline core that is made up of phosphate and iron oxide. Apoferritin binds to free ferrous iron and then it stores this in a ferric state. Free iron is toxic to cells and the body has developed an elaborate set of protective mechanisms in order to bind iron in a number of different tissue compartments. Within the cells, iron can be found to be stored complexed to protein as either a ferritin or hemosiderin.
Ferritin is synthesized in; the liver, spleen and many other body tissues. The measurement of serum levels can be carried out routinely. It is a useful indicator for the early detection of iron deficiency (anemia) in apparently healthy people. It is also regarded as clinically significant when monitoring the iron status for renal dialysis patients, blood donors and pregnant women. Early methods that were used to detect iron levels were lacking in sensitivity and very invasive (causing unnecessary trauma to patients). The current procedure to determine levels in serum offer better sensitivity and are non-invasive (very little patient discomfort).
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Zero Standard.
- Standard 1 – 5: Concentration 5 – 1000 ng/ml.
- Ferritin Control.
- Enzyme Conjugate: Consisting of antibody conjugated with horseradish peroxidase (HRP).
- Microtiterwells: Wells coated with ferritin antigen.
- TMB Substrate Solution.
- Stop Solution: Sulphuric acid (avoid any skin contact).
- Wash Solution (10x Concentrated).
SENSITIVITY
The minimum sensitivity detection limit of human ferritin using current ferritin ELISA kit was approximately 0.04 ng/ml. The dynamic range for this assay is 5.0 – 1000.0 ng/ml.
ASSAY CHARACTERISTICS
– Intra Assay Variation: Within ≤ 7.5%
– Inter Assay Variation: Within ≤ 6.1%
– Specificity: Liver Human Iso-Ferritin (100%), Spleen Human Iso-Ferritin (80%), Heart Human Iso-Ferritin (12%).
– Accuracy: Average value (±SD) of 98.66% ±2.90%, dilution test gave an average value (±SD) of 102.11% ± 5.32%.
– Sensitivity: 0.04 ng/mL at the 95 % confidence limit.
– Hook Effect: Shows no hook effect up to 50,000 ng/mL.
REFERENCES
- Ferritin as a Tumor Marker. J. Clin. Immunoassay (1983) 6/3: 234-240. Drysdale, J.W.
- Three Commercial Methods for Serum-Ferritin Compared and the High-Dose “Hook Effect” Eliminated. Clin. Chem. (1983) 29/6: 1109-1113, 1983. Ronald, Ng. H., et al.
- Human-ferritin for tumor detection and therapy. Wiley Interdiscip Rev Nanomed Nanobiotechnol. (2013) 5 (4): 287-98. Review. Fan K., Gao L. and Yan X.
ADDITIONAL INFORMATION
- Full Name: Ferritin ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma (EDTA, Heparin)
- Sensitivity: 0.04 ng/ml
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