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FeLV-gp70 (Feline Leukaemia Virus) ELISA Kit (Feline)

INTENDED USE

Feline FeLV-gp70 ELISA kit is a method for detecting feline leukemia virus gp70 (FeLV-gp70) antibodies present in either plasma or serum. This assay has a quantitative and qualitative standard range.

BACKGROUND

Feline leukemia virus (FeLV) is an infectious disease which is most common in cats. It can cause various blood disorders which can develop into leukaemia (cancer of the bone marrow), lymphoma (solid tumour of lymphocytes) and various other tumors. Due to this condition common viruses, bacteria, fungi and protozoa which usually did not affect healthy cats can now result in severe illness in FeLV infected cats. FeLV-gp70 is the glycoprotein of the envelope of FeLV. Following infection, antibodies against gp70 are produced by cats which have a neutralising effect.  It is widely accepted that a cat is protected if it has a neutralizing antibody titre above ± 32.

FELINE LEUKAEMIA VIRUS GP70 ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Strips. Coated with monoclonal anti-gp70 antibodies.
  • Negative Control.
  • Positive Control.
  • FeLV-gp70 Antigen.
  • Concentrated HRPO Conjugate.
  • Conjugate Buffer.
  • Substrate A.
  • Substrate B.
  • Strip Holder.
  • ELISA Buffer.
  • Wash Solution (200x Concentrated).
  • Stop Solution.
  • Plastic Cover Seal.

SENSITIVITY

The standard range of antibodies to feline leukemia virus (FeLV, feline leukemia virus) using current feline FeLV-gp70 ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Feline Leukemia Virus p70 (FeLV-gp70): ELISA
– The end point titer can be determined by comparing the OD of each dilution with the following threshold:
– OD negative control, at dilution 1:3 + 0.150.
– OD of a dilution is above this threshold the dilution is considered to be positive.
– Endpoint titers equal to or above 1:270 are considered to be protective in experimental situations.

REFERENCES

  1. Structure and self assembly of a retrovirus (FeLV) proline rich neutralization domain. J Biomol Struct Dyn. (1994) 11 (4): 821-36. Fontenot J.D., et al.
  2. Clearance of feline leukemia virus from persistently infected pet cats treated by extracorporeal immunoadsorption is correlated with an enhanced antibody response to FeLV gp 70. J Immunol. (1984) 132 (3): 1538-43. Snyder H.W. Jr, et al.
  3. Feline leukemia vaccine: efficacy, contents and probable mechanism. Vet Immunol Immunopathol. (1986) 11 (3): 205-13. Mastro J.M., et al.
  4. Control of feline leukaemia virus. Vet Immunol Immunopathol. (1989) 21 (1): 69-83. Review. Weijer K., et al.
  5. Appearance of cytotoxic antibody to viral gp70 on feline lymphoma cells (FL-74) in cats during ex vivo immunoadsorption therapy: quantitation, characterization, and association with remission of disease and disappearance of viremia. Proc Natl Acad Sci U S A. (1984) 81 (11): 3516-20. Liu W.T., et al.

ADDITIONAL INFORMATION

  • Full Name: FCV-gp70 (Feline Leukemia Virus) ELISA Kit (Feline)
  • Reactivity: Feline
  • Sample Type: Serum, Plasma
  • Sensitivity: Qualitative, Quantitative

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