FeLV-gp27 ELISA Kit (Feline Leukemia Virus)

Full Name: FeLV-gp27 ELISA Kit (Feline Leukemia Virus)
Reactivity: Feline
Sample Type: Serum, Plasma
Sensitivity: Qualitative

BACKGROUND

Feline leukemia virus (FeLV) is responsible for causing various blood disorder such as leukaemia and lymphoma in cats. If the virus is not defeated by the animal’s immune system then this could lead diseases which might be lethal. FeLV-A is a retrovirus with an RNA genome, meaning it is passed to offspring by the mother and can be passed on to other cats through contact with infected saliva or blood. It causes about 50% of all cases of feline leukaemia. Infection with FeLV-A is more likely to be congenital (occurring in the womb). In this case, the retrovirus was transmitted to the embryo and has persisted for generations in susceptible cats. No one knows how FeLV-A spreads from cat to cat. Feline leukemia virus Type A causes a disease called feline leukaemia or feline AIDS. Feline leukaemia is an often fatal cancer of white blood cells that attacks bone marrow, lymph nodes and spleen, leaving affected cats extremely vulnerable without treatment. Other causes may include chromosomal abnormalities, radiation exposure or some types of cancer treatment. Signs appear as anemia and weight loss, with blood tests indicating leukemia cells in the bone marrow and in lymph nodes.

One of the major core protein of FeLV is FeLV p27-antigen, this antigen can be found in the blood of infected felines. The following assay offers an alternative to the more widely used method IFA test on blood smears, with both procedures providing comparable results. We would also recommend carrying out an IFA, PCR or virus-isolation in order to confirm a positive ELISA result.

INTENDED USE

FeLV-gp27 ELISA kit is designed to detect feline leukemia virus-p27 antigen (FeLV-gp27) using serum and plasma samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Strips. Coated with monoclonal anti-FeLV-p27 antibodies.
Negative control (Freeze Dried).
Positive Control (Freeze Dried).
HRPO Conjugated Anti-Species Antibodies.
Substrate A.
Substrate B.
Strip Holder.
ELISA Buffer.
Wash Solution (200x Concentrated).
Plastic Cover Seal.
Stop Solution.

SENSITIVITY

The standard range of feline leukemia virus-p27 antigen using current FeLV-gp27 ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– FeLV postive control should give an OD (450nm) ≥ 1.000.
– Negative control must be lower OD (450nm) than 0.400.
– A sample is considered positive when the measured extinction is higher than 3 times the OD of the negative control.

REFERENCES

No benefit of therapeutic vaccination in clinically healthy cats persistently infected with feline leukemia virus. Vaccine. (2015) 33 (13): 1578-85. Helfer-Hungerbuehler A.K., et al.
Monoclonal antibodies to three epitopic regions of feline leukemia virus p27 and their use in enzyme-linked immunosorbent assay of p27. J Immunol Methods. (1983) 56 (2): 209-20. Lutz H., et al.
Expression of viral proteins in feline leukemia virus-associated enteritis. Vet Pathol. (2000) 37 (2): 129-36. Kipar A., et al.
Exposure of cats to low doses of FeLV: seroconversion as the sole parameter of infection. Vet Res. (2010) 41 (2): 17. Major A., et al.
Development and clinical evaluation of a rapid diagnostic kit for feline leukemia virus infection. J Vet Sci. (2014) 15 (1): 91-7. Kim W.S., et al.

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