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FCV (Feline Calici Virus) ELISA Kit (Feline)

INTENDED USE

Feline calici virus ELISA kit can be used for measuring antibodies against feline calici virus (FCV or feline calicivirus) in samples of serum or plasma. This assay has a quantitative and qualitative standard range.

BACKGROUND

Feline calicivirus (FCV) infection is a major cause of upper respiratory infections, which is more commonly known as cat flu. The virus is able to attack the respiratory tract, lungs, nasal passage and the mouth. It can also cause ulceration of the tongue, intestines and the musculoskeletal system. This is a ubiquitous virus and is responsible for causing the disease in cats throughout the world. At present there have been at least 40 different strains of FCV that have been discovered and the severity or the virulence of the disease resulting from the different strains can vary considerably. Even though vaccination against calicivirus is highly recommended, it seems to have failed in decreasing the prevalence of the disease. Some of the important things to consider during the diagnosis step include: clinical history, clinical signs, eye examination and laboratory findings. Method such as PCR and immunohistochemical staining have been useful in diagnosing this disease.

FELINE CALICI VIRUS ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Strips. Coated with FCV proteins.
  • Positive Control (Freeze Dried).
  • Negative control (Freeze Dried).
  • Strip Holder.
  • ELISA Buffer.
  • HRPO Conjugated Anti-Species Antibodies.
  • Wash Solution (200x Concentrated).
  • Substrate A.
  • Substrate B.
  • Plastic Cover Seal.
  • Stop Solution.

SENSITIVITY

The standard range of antibodies to feline calicivirus (FCV) using current feline calici virus (FCV) ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Feline Calici Virus (FCV): ELISA
– Weak positive control should give an extinction ≥ 1.500 OD and an endpoint titer ≥ 90.
– Negative control should give an OD ≤ 0.600 and an endpoint titer =< 30.
– A titer ≥ 270 is considered to be protective, under normal virus pressure.
– Summary:
(i). < 30 = no antibodies found.
(ii). > 90 = antibodies found, retest in 3 months. (probably shedding FCV)
(iii). > 270 = high titer, considered to be protective.

REFERENCES

  1. Inactivation of caliciviruses. Appl Environ Microbiol. (2004) 70 (8): 4538-43. Duizer E., et al.
  2. Feline inflammatory polyps: historical, clinical, and PCR findings for feline calici virus and feline herpes virus-1 in 28 cases. J Feline Med Surg. (2002) 4 (4): 195-9. Veir J.K., et al.
  3.  Treatment of cats with ocular disease attributable to herpesvirus infection: 17 cases (1983-1993). J Am Vet Med Assoc. (1995) 207 (5): 599-603. Stiles J.
  4. Chronic oral infections of cats and their relationship to persistent oral carriage of feline calici-, immunodeficiency, or leukemia viruses. Vet Immunol Immunopathol. (1991) 29 (1-2): 1-14. Tenorio A.P., et al.

ADDITIONAL INFORMATION

  • Full Name: FCV (Feline Calici Virus) ELISA Kit (Feline)
  • Reactivity: Feline
  • Sample Type: Serum, Plasma
  • Sensitivity: Qualitative, Quantitative

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