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FCoV (Feline Corona Virus) ELISA Kit (Feline)

INTENDED USE

Feline corona virus ELISA kit is intended for detecting antibodies against feline corona virus (FCoV)(mostly glycoproteins) in plasma, serum and ascetic fluid. This assay has a quantitative and qualitative standard range.

BACKGROUND

Feline coronavirus (FCoV) is infectious to cats throughout the world and it is caused by a positive stranded RNA. There are two different forms that exist: feline infectious peritonitis (FIP) and feline infectious peritonitis virus (FIPV). On the whole the majority of cats that are infected tend to recover fully and also develop immunity to corona virus. There is evidence that some cats can become carriers of the virus and therefore can infect other cats, however, there are a few cats which are unable to build immunity to corona virus and this can result in the disease developing into the fata form. This fatal form of FIP is a progressive, chronic disease which can be characterised by: loss of appetite, antibiotic-resistant fever, lethargy, anaemia and weakness. In majority of cases there is the presence of inflammation in the lining of the abdominal cavities and linings of the chest. Only a small number (approx. 5%) of the cats which are infected with the coronavirus actually develop the infectious peritonitis.

FELINE CORONA VIRUS ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Strips. Coated with FCoV proteins.
  • Strip Holder.
  • ELISA Buffer.
  • HRPO Conjugated Anti-Species Antibodies.
  • Positive Control (Freeze Dried).
  • Negative control (Freeze Dried).
  • Wash Solution (200x Concentrated).
  • Substrate A.
  • Substrate B.
  • Stop Solution.
  • Plastic Cover Seal.

SENSITIVITY

The standard range of antibodies to FCoV using current feline corona virus (FCoV) ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Feline Corona Virus (FCoV): ELISA
– Positive control should give an extinction > 0.800 OD units and an end point titer > 270.
– Negative control should give an OD < 0.400 and end point titer < 30.
– End point titer of the sample is the dilution that gives an extinction just above 0.350 OD units at 450 nm.

REFERENCES

  1. Utility of feline coronavirus antibody tests. J Feline Med Surg. (2015) 17 (2):152-62. Addie D.D., et al.
  2. Comparison of serologic techniques for the detection of antibodies against feline coronaviruses. J Vet Diagn Invest. (2008) 20 (1): 45-50. Pratelli A.
  3. Relevance of feline interferon omega for clinical improvement and reduction of concurrent viral excretion in retrovirus infected cats from a rescue shelter. Res Vet Sci. (2013) 94 (3): 753-63. Gil S., et al.
  4. Use of recombinant nucleocapsid proteins for serological diagnosis of feline coronavirus infection by three immunochromatographic tests. J Virol Methods. (2014) 196: 1-6. Takano T., et al.

ADDITIONAL INFORMATION

  • Full Name: FCoV (Feline Corona Virus) ELISA Kit (Feline)
  • Reactivity: Feline
  • Sample Type: Serum, Plasma, Ascetic Fluid
  • Sensitivity: Qualitative, Quantitative

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