Estrone ELISA Kit
- Created on the 15 February, 2017.
INTENDED USE
Human estrone ELISA kit is a reliable quantitative procedure for measuring estrone in human serum and plasma. This assay has a minimum sensitivity detection limit of 8.1 pg/ml.
BACKGROUND
Estrone is one of the major naturally occuring estrogens besides estriol and estradiol, it has a vital functions in developing female sex organs and secondary sex characteristics. Androstenedione is primarily responsible for the production of estrone. In men, postmenopausal women and prepubertal children the predominant portion of estrone can be derived from conversion of peripheral tissues. Also, more than 50% estrone can be secreted by the ovaries in premenopausal women. There are many bioassay data which are indicating that the estrogenic activity of estrone can be much lower when compared to that of estradiol.
REAGENTS SUPPLIED
Reagents which are supplied with the estrone ELISA kit, please following instructions for storage conditions.
1. Microtiter Plate: polyclonal anti-estrone antibody coated.
2. Standard (Standard 0-6): 0 – 15 – 30 – 90 – 270 – 810 – 2400 pg/mL.
3. Control Low & High.
4. Enzyme Conjugate.
5. Substrate Solution.
6. Stop Solution.
7. Wash Solution.
Note: Additional Standard 0 for sample dilution is available upon request.
SENSITIVITY
The minimum sensitivity detection limit of estrone using current human estrone ELISA kit was approximately 8.1 pg/ml. The dynamic range for this assay is 15.0 – 2,400.0 ng/ml.
REFERENCES
- Estrone sulfatase and its inhibitors. Anticancer Agents Med Chem. (2009) 9 (6): 599-612. Review. Aidoo-Gyamfi K., et al.
- Conjugation of estrone glucuronide with human lysozyme. J Immunoassay Immunochem. (2015) 36 (5): 532-46. Cooke D.G and Blackwell L.F.
- Estrone is neuroprotective in rats after traumatic brain injury. J Neurotrauma. (2012) 29 (12): 2209-19. Gatson J.W., et al.
ADDITIONAL INFORMATION
- Full Name: Estrone ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma
- Sensitivity: 8.1 pg/ml
