Rat Estradiol ELISA Kit (17-Beta Estadiol)

Full Name: Rat Estradiol ELISA Kit (17-Beta Estadiol)
Reactivity: Rat
Sample Type: Serum
Sensitivity: 2.5 pg/ml

BACKGROUND

Estradiol (also called 17β-estradiol, 17-beta estadiol, estradiol E2) is an estrogenic hormone with a molecular of 272.4 Da, it contains a phenolic A ring and is produced in the adrenal cortex, male testes, ovary and placenta. Majority (> 98%) of it that is present in the bloodstream circulates attached to the sex hormone binding globulin (SHBG) or albumin (lesser extent). This leaves a very small fraction to be circulation either as a free hormone or in the conjugated form.

Estradiol conjugated form is associated with more potent biological effects and accounts for the majority of the circulating hormone. It cannot develop secondary sex traits in men since it is a particular sort of steroid hormone. DHT, a byproduct of oestrogen, causes feminization or masculinization. In female rodents, the measurement of estradiol provides a vital marker for evaluating and monitoring essential reproductive functions and as well as pregnancy outcomes.

INTENDED USE

Rat estradiol ELISA kit can be used for determining quantitative amounts of estradiol (17-beta estadiol, estradiol-E2, 17β-estradiol) in rat serum samples. This assay has a minimum sensitivity detection limit of 2.5 pg/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Plate: Wells coated with anti-estradiol antibody.
• Calibrators (Calibrator 0-5): Lyophilized; concentrations: 0, 5, 20, 80, 320 and 1280pg/mL.
• Incubation Buffer.
• Enzyme Conjugate Diluent.
• Enzyme Conjugate (100x Concentrated).
• Substrate Solution (TMB).
• Stop Solution.
• Wash Solution (10x Concentrated).

SENSITIVITY

The minimum sensitivity detection limit of estradiol (estradiol E2, 17-beta estradiol, 17β-estradiol) using current rat estradiol ELISA kit was approximately 2.5 pg/ml. The dynamic range for this assay is 5.0 – 1,280.0 pg/ml.

ASSAY CHARACTERISTICS

– Analytical Sensitivity: 2.5 pg/mL
– Assay dynamic range: 2.5 – 1280 pg/mL
– Intra-Assay: 3.0 – 6.1%
– Inter-Assay: 4.0 – 7.1%
– Recovery: 81 – 117%
– Linearity: 82 – 117%

REFERENCES

1. Estradiol modulates effort-based decision making in female rats. Neuropsychopharmacology. (2012) 37 (2): 390-401. Uban K.A., et al.
2. Comparison of the effects of estradiol and progesterone on serotonergic function. Biol Psychiatry. (2012) 71 (7): 633-41. Benmansour S., et al.
3. Circulating sex hormones modulate vascular contractions and acute response to 17β-estradiol in rat mesenteric arteries. Pharmacology. (2011) 88 (1-2): 55-64. Keung W. and Man R.Y.
4. Sympathetic regulation of ovarian functions under chronic estradiol treatment in rats. Auton Neurosci. (2016) 197: 19-24. Uchida S. and Kagitani F.
5. Somatostatin inhibits prolactin secretion in the estradiol primed male rat. Can J Physiol Pharmacol. (1981) 59 (10): 1082-8. Cooper G.R. and Shin S.H.

OTHER RELATED ELISA KITS

• Estrone-3-Sulfate ELISA Kit (Equine)
• COX-2 ELISA Kit (Rat)
• Corticosterone ELISA Kit (Rat/Mouse)