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Estradiol E2 ELISA Kit

  • Created on the 15 February, 2017.

BACKGROUND

Estradiol (E2) is a steroid hormone that has a molecular weight of 272.4 Da, it contains a phenolic A ring. It is one of the most potent natural estrogen which can be produced predominately by the placenta, ovary, male testes and adrenal cortex. Over 98% of the estradiol (E2) that is secreted into the bloodstream is found to circulate attached to the sex hormone binding globulin (SHBG) and to a less extent to other proteins like albumin. This means that only a very small faction circulates in the conjugated form or as a free hormone form. There are estradiol-receptor complexes which are responsible for stimulating the appropriate response at the nuclear level within the specific the target sites. There are many target sites which can include: follicles, breast, uterus, urethra, vagina, pituitary, hypothalamus and to a lesser extent skin and liver.

In non-pregnant women with normal menstrual cycles, the secretion of estradiol is following a biphasic, cyclic pattern where the greatest levels are found just prior to ovulation. This can exert a positive feedback influence at the level of the pituitary where it can regulate the secretion of follicle stimulating hormone (FSH), luteinizing hormone (LH) and gonadotropins, all of which are vital for follicular maturation and ovulation. However, during pregnancy, the concentration of maternal serum estradiol levels is known to increase rapidly, to levels that are above the pre-ovulatory peak levels and these high levels are sustained throughout the pregnancy.

The determination of serum estradiol are essential in helping to evaluate a number of different menstrual dysfunctions for example precocious, menopause, delayed puberty in girls, primary and secondary amenorrhea. Elevated levels have also be identified in patients with gynaecomastia, testicular tumors and feminizing syndromes.

INTENDED USE

Human estradiol E2 ELISA kit is a reliable quantitative procedure for measuring estradiol E2 in human serum samples. This assay has a minimum sensitivity detection limit of 10.0 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of estradiol E2 using this human estradiol E2 ELISA kit was approximately 10.0 pg/ml. The dynamic assay range for this kit is 10.0 – 1,000.0 pg/ml.

REFERENCES

  1. Correlation between estradiol and progesterone in cycles with luteal phase deficiency. Fertil. Steril. (1982) 37: 348-354. Goldstein D., et al.
  2. The serum transport of steroid hormones, Rec. Prog. Horm. Res. (1982) 38: 457-510. Siiteri P.K., et al.
  3. Steroid biosynthesis by isolated human ovarian follicular cells in vitro, J. Clin. Endocrinol. Metab. (1980) 51: 1407-1411. Tsang B.K., Armstrong D.T. and Whitfield J.F.
  4. Oestradiol assays: applications and guidelines for the provision of a clinical biochemistry service, Ann. Clin. Biochem. (1988) 25:466-483. Ratcliffe W.A.,et al.

ADDITIONAL INFORMATION

  • Full Name: Estradiol E2 ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum
  • Sensitivity: 10.0 pg/ml

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