GET A QUOTE

Endothelin-3 (ET-3) ELISA Kit

  • Created on the 6 February, 2017.

BACKGROUND

Endothelin-3 (ET-3) is a member of the endothelin family of proteins and in humans is encoded by the EDN3 gene. These are essentially endothelium-derived vasoactive peptides which are involved in a number of different biological functions. The active form of this protein is a 21 amino acid sequence that is formed by the cleavage of the precursor protein. This is a ligand for endothelin receptor type B (EDNRB) and the interaction of this ligand with endothelin is essential for development of many neural crest-derived cell lineages for example enteric neurons and melanocytes. Any mutations in this gene and EDNRB are known to be linked to Waardenburg syndrome (WS) and Hirschsprung disease (HSCR), both of which are neural crest-derived cell congenital disorders.

INTENDED USE

Endothelin-3 ELISA kit is a reliable quantitative procedure for measuring endothelin-3 (ET-3) in human serum, tissue extracts, cell culture media and EDTA-plasma. This assay has a minimum sensitivity detection limit of 0.36 pg/ml.

SENSITIVITY

The minimum sensitivity detection limit of human endothelin-3 (ET-3) using this endothelin-3 ELISA kit was approximately 0.36 pg/ml. The dynamic assay range for this kit is 0.78 – 100.0 pg/ml.

REFERENCES

  1. Increased plasma levels of big-endothelin-2 and big-endothelin-3 in patients with end-stage renal disease. Life Sci. (2012) 91 (13-14): 729-32. Miyauchi Y., et al.
  2. Anatomically distinct activation of endothelin-3 and the L-arginine/nitric oxide pathway in the kidney with advanced aging. Biochem Biophys Res Commun. (2005) 327 (1): 234-41. Lattmann T., et al.
  3. Frequent loss of endothelin-3 (EDN3) expression due to epigenetic inactivation in human breast cancer. Breast Cancer Res. (2009) 11 (3): R34. Wiesmann F., et al.
  4. Endothelin-3 production by human rhabdomyosarcoma: a possible new marker with a paracrine role. Eur J Cancer. (2006) 42 (5): 680-7. Palladini A., et al.

ADDITIONAL INFORMATION

  • Full Name: Endothelin-3 (ET-3) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Tissue Extracts, EDTA-Plasma, Cell Culture Media
  • Sensitivity: 0.36 pg/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford