Endothelin-3 ELISA Kit (ET-3, EDN3)
INTENDED USE
Human endothelin-3 ELISA kit is a reliable quantitative procedure for measuring ET-3 (endothelin-3, EDN3) in human tissue extracts, cell culture media, serum and plasma (EDTA). This assay has a minimum sensitivity detection limit of 0.36 pg/ml.
BACKGROUND
Endothelin-3 (ET-3, EDN3) is a member of the endothelin family of proteins and in humans is encoded by the EDN3 gene. It is predominately produced in the gut, where the greatest levels are expressed in the caecum. There is evidence to indicate that the brain tissue displays an abundance of it, in the brain the main function involves the regulation of neuronal proliferation and development.
Endothelin-3 is formed from the proteolytic cleavage of the larger precursor protein by the endothelin-converting enzyme 1 (ECE1), this leads to the production of a 21 ammino acid peptide. It has a primary function of activating G-protein coupled molecules known as EDNRB. EDNRB is found to be highly expressed by gut mesenchyme and neural crest derived cells. This is a ligand for endothelin receptor type B (EDNRB) and the interaction of this ligand with endothelin-3 is essential for development of many neural crest-derived cell lineages for example enteric neurons and melanocytes.
Any mutations in this gene and EDNRB are known to be linked to Waardenburg syndrome (WS) and Hirschsprung disease (HSCR), both of which are neural crest-derived cell congenital disorders. The levels are found to be elevated in colon carcinoma, this is responsible for supporting cell survival and proliferation.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Precoated Microplate: Wells bound with ET-3 antigen 15-21 rabbit IgG affinity purify.
- Labelled Antibody (30x Concentrated): Consisting of HRP conjugated to endothelin-3 antibody.
- Standards: Concentration 0 – 100.0 pg/ml.
- EIA Buffer.
- Label Antibody Solution.
- TMB Chromogen Solution.
- Stop Solution.
- Wash Buffer (40x Concentrated).
SENSITIVITY
The minimum sensitivity detection limit of endothelin-3 (EDN3) using current human ET-3 ELISA kit was approximately 0.36 pg/ml. The dynamic range for this assay is 0.78 – 100.0 pg/ml.
REFERENCES
- Increased plasma levels of big endothelin-2 and big endothelin-3 in patients with end-stage renal disease. Life Sci. (2012) 91 (13-14): 729-32. Miyauchi Y., et al.
- Anatomically distinct activation of endothelin-3 and the L-arginine/nitric oxide pathway in the kidney with advanced aging. Biochem Biophys Res Commun. (2005) 327 (1): 234-41. Lattmann T., et al.
- Frequent loss of endothelin-3 (EDN3) expression due to epigenetic inactivation in human breast cancer. Breast Cancer Res. (2009) 11 (3): R34. Wiesmann F., et al.
- Endothelin-3 production by human rhabdomyosarcoma: a possible new marker with a paracrine role. Eur J Cancer. (2006) 42 (5): 680-7. Palladini A., et al.
ADDITIONAL INFORMATION
- Full Name: Endothelin-3 ELISA Kit (ET-3, EDN3)
- Reactivity: Human
- Sample Type: Cell Culture Media, Serum, Tissue Extracts, Plasma
- Sensitivity: 0.36 pg/ml
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