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Endothelin-2 (ET-2)(1-31) ELISA Kit

  • Created on the 6 February, 2017.

INTENDED USE

Endothelin-2 (1-31) ELISA kit is a reliable quantitative procedure for determining endothelin-2 (ET-2 [1-31]) in human serum, cell culture media, EDTA-plasma and tissue extracts. This assay has a minimum sensitivity detection limit of 4.0 pg/ml.

BACKGROUND

Endothelins belong to a family of isopeptides which includes big endothelin, endothelin-1, 2 and 3. They are characterised by having two essential disulfide bridges, a conserved amino acid sequence at the C-terminal and their potent vasoconstriction activity. Endothelins are produced by vascular endothelium cells which can be found in various tissues in vivo, such as pituitary, kidney, lung, placenta and brain.

Recently, a discovery of a novel smooth muscles constricting 31-amino acid endothelin was made, which was named endothelin-2 (1-31)(ET-2 [1-31]). This was found to be generated from the big endothelin using human chymase to cleave Tyr31 – Gly32 bond. The following endothelin-2 (1-31) ELISA kit is specific for ET-2 (1-31) and will not cross-react with ETs (1-21) and big ETs. However, it can be used to recognise both native and synthetic peptide forms of ET-2 (1-31).

SENSITIVITY

The minimum sensitivity detection limit of human ET-2 (1-31) using current endothelin-2 (1-31) ELISA kit was approximately 4.0 pg/ml. The dynamic range for this assay is 3.91 – 500.0 pg/ml.

REFERENCES

  1. Chymase processes big-endothelin-2 to endothelin-2-(1-31) that induces contractile responses in the isolated monkey trachea. Eur J Pharmacol. (1998) 358 (3): 229-33. Takai S, Shiota N, Jin D, Miyazaki M.
  2. Characterization of endothelin isoforms in human heart: endothelin-2 demonstrated. J Cardiovasc Pharmacol. (1993) 22 Suppl 8: S26-8. Plumpton C, et al.

ADDITIONAL INFORMATION

  • Full Name: Endothelin-2 (ET-2)(1-31) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, EDTA-Plasma, Cell Culture Media, Tissue Extracts
  • Sensitivity: 4.0 pg/ml

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