ENA Screen ELISA Kit

  • Created on the 2 June, 2017.


Human ENA screen ELISA kit can be used for in-vitro quantitative screening of IgG auto-antibodies directed against extractable nuclear antigens (ENAs) in human serum and plasma samples. This assay has a minimum analytical sensitivity limit of diagn. 92.7%.

A mixture of purified antigens SS-A 60, SS-A 52, SS-B, Sm, RNP/Sm, Scl 70 and Jo-1 is coated on to microwells.


Extractable nuclear antigens (ENA) are predominately linked to connective tissue disorders (CTD), they are soluble cytoplasmic and nuclear antigens. Over 100 different types have been identified, however, there are 6 main groups which are frequently used in research laboratories in order to investigate Sjögren’s syndrome, systemic lupus erythematosus (SLE) and mixed connective disease (MCTD). These are anti-La and anti-Ro for Sjögren’s, anti-Scl70 and anti-Jo for Dermatomyositis, anti-Sm for SLE and anti-RNP for MCTD. The sensitivity and specificity generated for these procedures will depend on the assay or test that is being carried out and this will there vary between each laboratory. The following ENA screen ELISA kit is an ideal for many diagnostic applications.


The minimum detection sensitivity level of IgG antibodies directed to human extractable nuclear antigens (ENAs) to screen using current human ENA screen ELISA kit was diagn. 92.7%. The dynamic range for this assay is to a cut-off index 1.0.


  1. Efficiency of different strategies to detect autoantibodies to extractable nuclear antigens. J Immunol Methods. (2010) 360 (1-2): 89-95. Almeida González D., et al.
  2. Comparison of two extractable nuclear antigen testing algorithms: ALBIA versus ELISA/line immunoassay. Pathology. (2016) 48 (5): 491-7. Chandratilleke D., et al.
  3. How should a district general hospital immunology service screen for anti-nuclear antibodies? An ‘in-the-field’ audit. Clin Exp Immunol. (2015) 180 (1): 52-7. Hira-Kazal R., et al.
  4. Application of linear discriminant analysis in performance evaluation of extractable nuclear antigen immunoassay systems in the screening and diagnosis of systemic autoimmune rheumatic diseases. Am J Clin Pathol. (2012) 138 (4): 596-603. Pi D., et al.


  • Full Name: ENA Screen ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: 92.7%



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