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ENA Screen ELISA Kit

  • Created on the 2 June, 2017.

BACKGROUND

Extractable nuclear antigens (ENA) are predominately linked to connective tissue disorders (CTD), they are soluble cytoplasmic and nuclear antigens. Over 100 different types have been identified, however, there are 6 main groups which are frequently used in research laboratories in order to investigate Sjögren’s syndrome, systemic lupus erythematosus (SLE) and mixed connective disease (MCTD). These are anti-La and anti-Ro for Sjögren’s, anti-Scl70 and anti-Jo for Dermatomyositis, anti-Sm for SLE and anti-RNP for MCTD. The sensitivity and specificity generated for these procedures will depend on the assay or test that is being carried out and this will there vary between each laboratory.

INTENDED USE

Human ENA ELISA kit can be used for in-vitro quantitative screening of IgG autoantibodies directed against extractable nuclear antigens (ENAs) in human serum and plasma samples. This assay has a minimum analytical sensitivity limit of diagn. 92.7%.

A mixture of purified antigens SS-A 60, SS-A 52, SS-B, Sm, RNP/Sm, Scl 70 and Jo-1 is coated on to microwells.

SENSITIVITY

The minimum detection sensitivity level of human extractable nuclear antigens (ENAs) using this human ENA ELISA kit was diagn. 92.7%. The dynamic assay range for this kit is to a cut-off index 1.0.

REFERENCES

  1. Efficiency of different strategies to detect autoantibodies to extractable nuclear antigens. J Immunol Methods. (2010) 360 (1-2): 89-95. Almeida González D., et al.
  2. Comparison of two extractable nuclear antigen testing algorithms: ALBIA versus ELISA/line immunoassay. Pathology. (2016) 48 (5): 491-7. Chandratilleke D., et al.
  3. How should a district general hospital immunology service screen for anti-nuclear antibodies? An ‘in-the-field’ audit. Clin Exp Immunol. (2015) 180 (1): 52-7. Hira-Kazal R., et al.
  4. Application of linear discriminant analysis in performance evaluation of extractable nuclear antigen immunoassay systems in the screening and diagnosis of systemic autoimmune rheumatic diseases. Am J Clin Pathol. (2012) 138 (4): 596-603. Pi D., et al.

ADDITIONAL INFORMATION

  • Full Name: ENA Screen ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: 92.7%

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