- Created on the 2 June, 2017.
Extractable nuclear antigens (ENA) are nuclear and soluble cytoplasmic antigens which are associated with connective tissue disorders (CTD). There are over 100 different types, however, the six main antigens which are frequently utilised in immunological laboratories are Sm, Scl-70, Ro, Jo1, La and RNP. These are usually intended to screen for mixed connective disease, systemic lupus erythematosus and Sjögren’s syndrome for example anti-Sm (for SLE), anti-RNP (for MCTD), anti-Ro and anti-La (for Sjögren’s), anti-Jo and anti-Scl70 (for Dermatomyositis). The specificity and sensitivity obtained used these test will depend largely on the type of assay being employed and therefore vary from on laboratory to another.
Human ENA ELISA kit for semi-quantitative screening of IgG extractable nuclear antigens (ENAs) in human serum and plasma samples. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.
Purified antigens SS-A (52 and 60 kDa), SS-B, Sm, RNP/Sm, Scl-70 and Jo-1 are bound to individual rows of the microwell plate.
The minimum detection sensitivity level of human extractable nuclear antigens (ENAs) using this human ENA ELISA kit was 0.5 U/ml. The dynamic assay range for this kit is 12.5 – 100.0 U/ml.
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- Full Name: ENA Combi ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma
- Sensitivity: 0.5 U/ml