GET A QUOTE

ENA Combi ELISA Kit

  • Created on the 2 June, 2017.

BACKGROUND

Extractable nuclear antigens (ENA) are nuclear and soluble cytoplasmic antigens which are associated with connective tissue disorders (CTD). There are over 100 different types, however, the six main antigens which are frequently utilised in immunological laboratories are Sm, Scl-70, Ro, Jo1, La and RNP. These are usually intended to screen for mixed connective disease, systemic lupus erythematosus and Sjögren’s syndrome for example anti-Sm (for SLE), anti-RNP (for MCTD), anti-Ro and anti-La (for Sjögren’s), anti-Jo and anti-Scl70 (for Dermatomyositis). The specificity and sensitivity obtained used these test will depend largely on the type of assay being employed and therefore vary from on laboratory to another.

INTENDED USE

Human ENA ELISA kit for semi-quantitative screening of IgG extractable nuclear antigens (ENAs) in human serum and plasma samples. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.

Purified antigens SS-A (52 and 60 kDa), SS-B, Sm, RNP/Sm, Scl-70 and Jo-1 are bound to individual rows of the microwell plate.

SENSITIVITY

The minimum detection sensitivity level of human extractable nuclear antigens (ENAs) using this human ENA ELISA kit was 0.5 U/ml. The dynamic assay range for this kit is 12.5 – 100.0 U/ml.

REFERENCES

  1. Autoantibodies to extractable nuclear antigens: making detection and interpretation more meaningful. Clin Diagn Lab Immunol. (2002) 9 (1): 1-7. Review. Phan T.G., et al.
  2. From ANA to ENA: how to proceed? Autoimmun Rev. (2006) 5 (1): 10-7. Review. Damoiseaux J.G. and Tervaert J.W.
  3. Serum antinuclear and extractable nuclear antigen antibody prevalence and associated morbidity and mortality in the general population over 15 years. Autoimmun Rev. (2016) 15 (2): 162-6. Review. Selmi C., et al.
  4. The use of laboratory tests in diagnosis and monitoring of systemic lupus erythematosus. J Nephrol. (2002) 15 Suppl 6: S20-7. Review. Sinico R.A., et al.
  5. Antibodies targeting extractable nuclear antigens: historical development and current knowledge. Br J Dermatol. (2001) 145 (6): 859-67. Review. Wenzel J., et al.

ADDITIONAL INFORMATION

  • Full Name: ENA Combi ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.5 U/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford