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Elastase ELISA Kit

  • Created on the 2 June, 2017.

BACKGROUND

Elastase is part of the subfamily serine protease that hydrolyze many proteins such as elastin, it is formed as an inactive zymogen (within the pancreas) and becomes active by trypsin (within the duodenum). In humans, there are six elastase genes that encode structurally similar proteins (elastase 1, 2, 2A, 2B, 3A, 3B). Neutrophil elastase is one of the main protease enzyme, it is 218 amino acids I length and contains two asparagine-linked carbohydrate chains that are important for glycosylation. They are able to hydrolyzes proteins within specialized neutrophil lysosomes (known as azurophil granules) or extracellular matrix proteins and when neutrophil elastase is expressed aberrantly there is evidence that it can cause emphysematous and emphysema changes. It mainly occurs in polymorph nuclear neutrophil granulocytes (PMN) in endothelial and macrophages cells and it is also significantly involved in tissue destruction which are associated to rheumatoid arthritis and emphysema, where autoantibodies targeted against elastase tend to be linked with inflammatory rheumatic disorders such as systemic lupus erythematosus, rheumatoid arthritis and vasculitis. Elastase and proteinase 3 display a sequence homology of 54 %.

INTENDED USE

Human elastase ELISA kit is designed for measuring in vitro quantitative levels of IgG autoantibodies to elastase in human plasma and serum. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human elastase using this human elastase ELISA kit was 0.5 U/ml. The dynamic assay range for this kit is 6.3 – 100.0 U/ml.

REFERENCES

  1. Platelets, Micro-Particles and Elastase. A Review with Extrapolation to the Mechanism of Generation and Bio-Pathology of Platelet Fragments. Pathol Oncol Res. (2017) 23 (2): 455-458. Robert L. and Labat-Robert J.
  2. Interaction between elastin and elastases and its role in the aging of the arterial wall, skin and other connective tissues. A review. Mech Ageing Dev. (1984) 28 (2-3): 155-66. Review. Robert L., et al.
  3. Elastase-induced pulmonary emphysema: insights from experimental models. An Acad Bras Cienc. (2011) 83 (4): 1385-96. Review. Antunes M.A. and Rocco P.R.
  4. Anti-inflammatory activity of neutrophil elastase inhibitors. Curr Opin Investig Drugs. (2003) 4 (5): 556-65. Review. Tremblay G.M., et al.

ADDITIONAL INFORMATION

  • Full Name: Elastase ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.5 U/ml

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