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E-Selectin (CD62E) ELISA Kit

  • Created on the 3 May, 2017.

BACKGROUND

E-selectin (also called LECAM2, ELAM-1 and CD62E) is only expressed in endothelial cells that have activated by cytokines and it is a cell adhesion molecules which consists of 6 sushi domain units, EDF-like domain, transmembrane domain, intracellular cytoplasmic tail, C-type lectin domain and an N-terminal. The expression of p-selectin is responsible for stimulating the production of E-selectin which in turn is stimulated by IL-1, TNF-alpha and lipopolysaccharide. Like many of the other selectins, it is also responsible in playing vital roles during inflammation – it is important in recruiting leukocytes to the site of injury. Cytokine activated endothelial cells that are expressing E-selectin have the ability to bind CD44, PSGL-1 and many other ligands which are unidentified. There are also many findings to indicate that E-selectin is able to mediate the adhesion of tumor cells to endothelial cells. It is also fast becoming a popular biomarker for the metastatic potential of a number of cancers such as colorectal cancer.

INTENDED USE

Human E-selectin ELISA kit is designed for detecting in vitro quantitative levels of e-selectin (ELAM-1, CD62E, LECAM2) in human serum, cell culture supernatant, plasma, other biological fluids and cell lysates. This assay has a minimum sensitivity limit of 2.51 ng/ml.

SENSITIVITY

The minimum detection sensitivity level of CD62E, ELAM-1 or LECAM2 using this human E-selectin ELISA kit was 2.51 ng/ml. The dynamic assay range for this kit is 5.0 – 2,000 ng/ml.

REFERENCES

  1. E-selectin ligands as mechanosensitive receptors on neutrophils in health and disease. Ann Biomed Eng. (2012) 40 (4): 849-59. Review. Chase S.D., et al.
  2. Leukocyte ligands for endothelial selectins: specialized glycoconjugates that mediate rolling and signaling under flow. Blood. (2011) 118 (26): 6743-51. Review. Zarbock A., et al.
  3. Are selectins involved in metastasis? Clin Exp Metastasis. (1999) 17 (3): 183-92. Review. Krause T. and Turner G.A.
  4. E-selectin as a target for drug delivery and molecular imaging. J Control Release. (2012) 158 (2): 194-206. Review. Jubeli E., et al.
  5. Selectins promote tumor metastasis. Semin Cancer Biol. (2010) 20 (3): 169-77. Review. Läubli H. and Borsig L.
  6. Targeting selectins and selectin ligands in inflammation and cancer. Expert Opin Ther Targets. (2007) 11 (11): 1473-91. Review. Barthel S.R., et al.

ADDITIONAL INFORMATION

  • Full Name: E-Selectin (CD62E) ELISA Kit
  • Reactivity: Human
  • Sample Type: Cell Lysates, Serum, Cell Culture Supernatant, Plasma, Other Biological Fluids
  • Sensitivity: 2.51 ng/ml

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