GET A QUOTE

DMP1 (Dentin Matrix Protein 1) ELISA Kit (Rat)

  • Created on the 3 November, 2017.

INTENDED USE

Rat DMP1 ELISA kit is designed for detecting quantitative levels of dentin matrix protein 1 (DMP1, DMP-1) in rat plasma, serum and cell culture supernatant. This assay has a minimum sensitivity detection limit of 4.04 pg/ml.

BACKGROUND

Dentin matrix protein 1 (DMP1) is a protein that is vital for dentin and proper mineralisation of bone, it can be found in a wide range of cells of tooth and bone tissues. A member of the Small Integrin-Binding Ligand, N-linked Glycoprotein (SIBLING) family and a constitutive protein that is present within the extracellular matrix of osteocytes. Initially synthesised as 105kDa proprotein (full length) and then divided into 57kDa C terminal protein fragment and a 37kDa N terminal protein fragment just before it is being secreted. The protein itself consists multiple phosphorylation sites acidic domains, a large number of acidic domains, a DNA binding domain and a functional arg-gly-asp cell attachment sequence. DMP1 in the blood is regarded as a crucial biomarker that can reflect osteocyte activity, provide useful information on the process of bone and phosphate metabolism. Mutations in the gene are found to lead to autosomal recessive hypophosphatemia, this is a disease which results in osteomalacia and rickets.

SENSITIVITY

The minimum sensitivity detection limit of dentin matrix protein 1 (DMP-1) using current rat DMP1 ELISA kit was approximately 4.04 pg/ml. The dynamic range for this assay is 39.06 – 2,500.0 pg/ml.

REFERENCES

  1. Novel sandwich ELISAs for rat DMP1: age-related decrease of circulatory DMP1 levels in male rats. Bone. (2013) 57 (2): 429-36. Sato S., et al.
  2. Transcriptional regulation of dentin matrix protein 1 (DMP1) in odontoblasts and osteoblasts. Connect Tissue Res. (2014) 55 Suppl 1: 107-12. Jacob A., et al.
  3. Possible role of DMP1 in dentin mineralization. J Struct Biol. (2011) 174 (1): 100-6. Beniash E., et al.
  4. Distribution of small integrin-binding ligand, N-linked glycoproteins (SIBLING) in the condylar cartilage of rat mandible. Int J Oral Maxillofac Surg. (2010) 39 (3): 272-81. Sun Y., et al.
  5. Recombinant expression and characterization of dentin matrix protein 1. Connect Tissue Res. (1999) 40 (4): 251-8. Srinivasan R., et al.
  6. Sequential expression of matrix protein genes in developing rat teeth. Matrix Biol. (1999) 18 (2): 133-43. Bleicher F., et al.

ADDITIONAL INFORMATION

  • Full Name: DMP1 (Dentin Matrix Protein 1) ELISA Kit (Rat)
  • Reactivity: Rat
  • Sample Type: Plasma, Cell Culture Supernatant, Serum
  • Sensitivity: 4.04 pg/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford