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Deoxynivalenol ELISA Kit

  • Created on the 5 October, 2017.

BACKGROUND

Deoxynivalenol (also referred to as DON or vomitoxin) is an epoxy-sesquiterpenoid, a particular type of B trichothecene. It is usually found to co-exist with a number of other toxins which are produced by the same organism that is able produce this toxin, (in particular especially zearalenone). This mycotoxin is present predominantly in grains (for example barley, wheat, oats, corn and rye) and to a less extend in sorghum, triticale and rice. The principal DON-producing fungus in grains is Fusarium graminearum, small grains (such as oats, wheat and barley) and corn are often then major crops that become affected. In human foods, vomitoxin is not recognised as a carcinogen, this is in contrast to aflatoxin. Therefore, large amounts of grain containing vomitoxin needs to be consumed in order to pose any health risk to humans. Deoxynivalenol is regarded as a gastrointestinal irritant and also an inhibitor in protein synthesis. The ELISA assay provides a highly sensitive method which is capable of rapid quantification of deoxynivalenol contaminations present in beer or in cereals.

INTENDED USE

Deoxynivalenol (DON) ELISA kit is designed for analysing rapid quantitative levels of deoxynivalenol (DON or vomitoxin) present in cereals and gyle/beer. This assay has a minimum analytical sensitivity limit of 0.08 ppm.

SENSITIVITY

The minimum detection sensitivity level of vomitoxin (deoxynivalenol) using this deoxynivalenol (DON) ELISA kit was 0.08 ppm. The standard assay range for this kit is 0.2 – 5.0 ppm.

REFERENCES

  1. Correlation of deoxynivalenol and fumonisin concentration determined in maize by ELISA methods. J Immunoassay Immunochem. (2012) 33 (4): 414-21. Pleadin J., et al.
  2. Production of anti-idiotype antibodies for deoxynivalenol and their evaluation with three immunoassay platforms. Mycotoxin Res. (2014) 30 (2): 103-11. Maragos C.M.
  3. Enzyme-linked immunosorbent assayin analysis of deoxynivalenol: investigation of the impact of sample matrix on results accuracy. Anal Bioanal Chem. (2014) 406 (2): 505-14. Dzuman Z., et al.
  4. Modified use of a commercial ELISA kit for deoxynivalenol determination in rice and corn silage. Mycotoxin Res. (2013) 29 (2): 79-88. Hiraoka H., et al.
  5. Deoxynivalenol in commercial beer – screening for the toxin with an indirect competitive ELISA. Mycotoxin Res. (1993) 9 (2): 99-109. Niessen L., et al.

ADDITIONAL INFORMATION

  • Full Name: Deoxynivalenol ELISA Kit
  • Sample Type: Beer/Gyle, Cereals
  • Sensitivity: 0.08 ppm

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PhD Student / University of Glasgow

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Lecturer / University College London (UCL)

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Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

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Lead Scientists / AstraZeneca

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Purchasing / University of Oxford