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Dehydroepiandrosterone (DHEA) ELISA Kit

INTENDED USE

Human dehydroepiandrosterone ELISA kit is a reliable quantitative procedure for measuring DHEA (dehydroepiandrosterone, androstenolone) in human serum and plasma (EDTA) samples. This assay has a minimum sensitivity detection of 0.07 ng/ml.

BACKGROUND

Dehydroepiandrosterone (DHEA, 3b-hydroxy-5-androsten-17-one, androstenolone) is predominately produced in the adrenal cortex and to a smaller amount in the gonads, it is a C19 steroid which serves as a precursor for the synthesis of testosterone and estrogen. The androgenic activity of DHEA is relatively weak in comparison to testosterone due to the presence of a 17-oxo instead of a hydroxyl group. However, there is literature to indicate that in adult women, peripubertal children and in neonates the levels of circulating dehydroepiandrosterone can be many fold higher than testosterone concentrations and that there is a possibility of a rapid peripheral tissue conversion to more potent androgens (such as testosterone  and androstenedione) and estrogens.

Currently, the exact role of DHEA is not conclusive and there are a number of in vivo effects which have been reported such as enzyme-mediated cell metabolism and antiproliferative effects in different cell lines. There has been abnormal dehydroepiandrosterone levels which have been identified in patients with obesity and schizophrenia, also therapeutic administration of human dehydroepiandrosterone have been proposed for a number conditions like obesity and cardiovascular diseases. In vivo studies have indicated that DHEA could affect insulin sensitivity and secretion, lipid and cholesterol metabolism and immune function. The following DHEA ELISA kit is suitable for analysing either serum or plasma samples. The determination of serum dehydroepiandrosterone has become a useful marker of adrenal androgen synthesis.  Abnormally low have been identified in hypoadrenalism and elevated concentrations are found in many conditions such as: female hirsutism, 21-hydroxylase and 3b-hydroxysteroid dehydrogenase deficiencies and in most virilizing adrenal adenoma and carcinoma. Also due to the fact that very small amount of DHEA is produced by the gonads, by determining the levels of DHEA could help in localizing the androgen source in virilizing conditions.

DHEA ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Break apart strips coated with DHEA antigen.
  • Enzyme Conjugate (100x Concentrate): Contains dehydroepiandrosterone antibody conjugated to HRP.
  • Standards A-F: Concentration 0-2500 pg/ml. Avoid multiple freezing and thawing cycles.
  • Control (High) and Control (Low): Containing human DHEA antibody in protein-based buffer.
  • TMB Substrate Solution.
  • Stop Solution: 1 M H2SO4.
  • Wash Buffer Concentrate (10 x).
  • Protein Assay Buffer.

SENSITIVITY

The minimum sensitivity detection limit of dehydroepiandrosterone (DHEA, androstenolone) using current human DHEA ELISA kit was approximately 0.07 ng/ml. The dynamic range for this assay is 0.3 – 30.0 ng/ml.

REFERENCES

  1. Dehydroepiandrosterone (DHEA): A fountain of youth? .Journal of Clinical Endocrinology and Meatabolism, (1996) 18: 3147. Baulieu, E.M.
  2. DHEA therapy for women: effect on sexual function and wellbeing. Hum Reprod Update. (2007) 13 (3): 239-48. Review. Panjari M. and Davis S.R.
  3. Dehydroepiandrosterone (DHEA): a steroid with multiple effects. Is there any possible option in the treatment of critical illness? Curr Med Chem. (2010) 17 (11): 1039-47. Review. Oberbeck R. and Kobbe P.

ADDITIONAL INFORMATION

  • Full Name: Dehydroepiandrosterone (DHEA) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma (EDTA)
  • Sensitivity: 0.07 ng/ml

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