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Creatine Kinase MB (CK-MB) ELISA Kit

  • Created on the 16 May, 2017.

BACKGROUND

Creatine kinase (CK) which was previously known as creatine phosphokinase, is an intracellular enzyme that is found in heart, brain and muscles. It is known to exit as three different isoenzymes CK-MB, CK-MM and CK-BB. CK-MB is most frequently found in the muscles of the heart, CK-MM can be found in the skeletal and voluntary muscles of the body and CK-BB is found in the brain, smooth and involuntary muscles of the body.

CK-MB is released into blood when there is damage to the heart muscles, for example during a heart attack, the levels of CK-MB rise around 3-6 hours after the onset of chest pains and they peak at 12-24 and the return to normal levels around 48-72 hours. This makes it a useful marker for the heart in order to identify if any trauma or injury has taken place. However, a troponin test is normally the first choice since it is more specific for damage to the heart but if this is not available then CK-MB is considered an acceptable substitute.

INTENDED USE

Human CK-MB ELISA kit is intended for detecting in vitro quantitative concentrations of creatine kinase MB (CK-MB) in human cell culture supernatant, serum, plasma, other biological fluids and cell lysates. This assay has a minimum sensitivity limit of 1.11 ng/ml.

SENSITIVITY

The minimum detection sensitivity level of human CK-MB using this human creatine kinase MB ELISA kit was 1.11 ng/ml. The dynamic assay range for this kit is 0.2 – 60.0 ng/ml.

REFERENCES

  1. The clinical utility of CK-MB measurement in patients suspected of acute coronary syndrome. Clin Chim Acta. (2016) 456: 89-92. Kim J. and Hashim I.A.
  2. Isolated creatine kinase-MB rise with normal cardiac troponins: a strange occurrence with difficult interpretation. J Cardiovasc Med (Hagerstown). (2011) 12 (10): 736-40. Review. Ricci F. and De Caterina R.
  3. A tale of two biomarkers: the use of troponin and CK-MB in contemporary practice. Clin Lab Sci. (2010) 23 (3): 134-40. Review. Saenger A.K.
  4. Elevated cardiac biomarkers with normal right ventricular size indicate an unlikely diagnosis of acute pulmonary embolism in stable patients. Clin Appl Thromb Hemost. (2011) 17 (6): E153-7. Stein P.D., et al.
  5. Laboratory diagnosis of patients with acute chest pain. Clin Chem Lab Med. (2000) 38 (3): 187-97. Review. Penttilä I., et al.

ADDITIONAL INFORMATION

  • Full Name: Creatine Kinase MB (CK-MB) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Cell Culture Supernatant, Other Biological Fluids, Plasma, Cell Lysates
  • Sensitivity: 1.11 ng/ml

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