CPV IgM ELISA Kit (Canine Parvovirus)

Full Name: CPV IgM ELISA Kit (Canine Parvovirus)
Reactivity: Canine
Sample Type: Plasma, Serum
Sensitivity: Qualitative

BACKGROUND

Canine parvovirus (CPV), exists in two forms the intestinal and cardiac form, it is a viral infections that commonly infects dogs. The rate of incidence for this infection has been radically reduced with the help of early vaccination of puppies. Intestinal CPV, infects the lining of the intestinal tract and its symptoms are vomiting, diarrhea and lethargy. Cardiac form of this disease is more serious and can lead to congestive heart failure or death within days. Common signs are lethargy that leads to collapse and rapid breathing followed by cardiac arrest. There is no specific treatment for canine parvovirus but supportive therapy can be used such as fluid therapy and antibiotics in severe cases.

CPV infections are the result of genetic alterations to the original canine parvovirus type 2b and these new parvovirus are able to spread either by direct contact or indirectly through faecal-oral. The following method is frequently used to measure the antibody titre of the infected virus.

INTENDED USE

CPV IgM ELISA kit can be used to detect IgM antibodies to canine parvovirus (CPV IgM) in serum and plasma.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Strips: Coated with monoclonal anti parvo virus antibody.
• Strip Holder.
• Anti-Dog IgM HRPO Conjugate.
• ELISA Buffer.
• Wash Solution (10x Concentrated).
• Parvo Antigen Solution.
• Positive Control Serum (Freeze-Dried).
• Negative Control (Freeze-Dried).
• Substrate A.
• Substrate B.
• Stop Solution.
• Plastic Cover Seal.

SENSITIVITY

The standard range of IgM antibodies directed against canine parvovirus (CPV IgM) using current CPV IgM ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Negative control should give an OD < 0.350.
– End point titer of the positive control should be between 1:150 and 1:450.
– Test is valid if the first two dilutions of the positive control are above 0,350 (OD 450 nm).

REFERENCES

1. Evaluation of a dot ELISA kit for measuring immunoglobulin M antibodies to canine parvovirus and distemper virus. Vet Rec. (2003) 152 (19): 588-91. Waner T., et al.
2. Development of a duplex rapid assay for immunoglobulins M and G to evaluate the parvoviral immune status of clinically healthy dogs. J Vet Diagn Invest. (2016) 28 (3): 299-303. Palma M., et al.
3. Clinical and pathologic features of parvoviral diarrhea in pound-source dogs. J Am Vet Med Assoc. (1984) 185 (6): 651-5. Stann S.E., et al.
4. Systemic and local intestinal antibody response in dogs given both infective and inactivated canine parvovirus. Am J Vet Res. (1983) 44 (11): 1989-95. Nara P.L., et al.

OTHER RELATED ELISA KITS

• CPV (Canine Parvovirus) IgG ELISA Kit (Dog)
• CAV (Canine Adenovirus) Ab ELISA Kit (Dog)
• CCV (Canine Coronavirus) IgM ELISA Kit (Dog)