CPV IgG ELISA Kit (Canine Parvovirus)

Full Name: CPV IgG ELISA Kit (Canine Parvovirus)
Reactivity: Canine
Sample Type: Serum, Plasma
Sensitivity: Qualitative

BACKGROUND

Canine parvovirus (CPV) is a contagious viral infection that affects dogs, which can exist in two different forms. The intestinal form is the most common, characterised by weight loss, lack of appetite, anorexia and vomiting. The cardiac form is the less common, this can attack the heart muscles of very young puppies often resulting in death. Majority of cases for this infection have been identified in puppies between 6 weeks to 6 months old. CPV infections for majority of the time are caused by a genetic alteration of the original canine parvovirus. The fecal-oral route or direct contact with an infected dog are the two ways that these parvoviruses might spread. The infection incidence rates have been radically reduced through early vaccination of young puppies.

CPV can be diagnosed with a physical examination, abdominal radiographs/ultrasounds, biochemical tests and urine analysis. Also, for diagnosis of canine parvovirus infection or vaccination control another common used procedure includes analysing antibody titre. The following procedure is composed of the virus that is able to attach to the solid phase by using specific monoclonal antibodies.  IgG antibody titers that are above a dilution of 1:810 are frequently regarded as being protected.

INTENDED USE

CPV IgG ELISA kit is designed to determine quantitative amounts of IgG antibodies directed against canine parvovirus (CPV IgG) in samples of plasma or serum.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Coated Microtiter Strips.
• Strip Holder.
• Inactivated Canine Parvo Virus Antigen.
• ELISA Buffer.
• HRPO Conjugate.
• Positive Control (Freeze Dried).
• Negative Control (Freeze Dried).
• Wash Solution (200x Concentrated).
• Substrate Buffer A.
• Substrate Buffer B.
• Stop Solution.
• Cover Seal.

SENSITIVITY

The standard range of IgG antibodies to canine parvovirus (CPV-IgG) using current CPV IgG ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Negative control (at the 1:30 dilution) should give an OD < 0.400.
– End point titer of the positive control should be between 1:150 and 1:450.
– The test is valid if the first two dilutions of the positive control are above 0.500 OD (450 nm).
– In Summary:
(i). 30: = No antibodies found.
(ii). 90-270: = Antibodies found.
(iii). ≥ 810: = High titer of antibodies found.

REFERENCES

1. Evaluation of Immunity and Seropositivity of IgG Antibodies to Canine Parvoviruses in Vaccinated and Unvaccinated Dogs in Abeokuta, Nigeria. J Immunoassay Immunochem. (2016) 37 (1): 16-28. Babalola E.T., et al.
2. Nucleic acid immunization protects dogs against challenge with virulent canine parvovirus. Vaccine. (1998) 16 (6): 601-7. Jiang W., et al.
3. Systemic and local intestinal antibody response in dogs given both infective and inactivated canine parvovirus. Am J Vet Res. (1983) 44 (11): 1989-95. Nara P.L., et al.
4. Magnetic protein microbead-aided indirect fluoroimmunoassay for the determination of canine virus specific antibodies. Biosens Bioelectron. (2011) 26 (7): 3353-60. Wang X., et al.

OTHER RELATED ELISA KITS

• CCV (Canine Coronavirus) IgM ELISA Kit (Dog)
• CCV (Canine Coronavirus) IgM ELISA Kit (Dog)
• CAV (Canine Adenovirus) Ab ELISA Kit (Dog)