GET A QUOTE

COX-2 ELISA Kit (Rat)

  • Created on the 2 November, 2017.

BACKGROUND

Cyclooxygenase-2 (COX2) is also called prostaglandin G/H synthase 2 (PGHS2), it is an enzyme that is membrane bound that is important in the catalysis of prostanoid synthesis. It is responsible for the forming of prostanoid which can include prostaglandins and thromboxane (for example prostacyclin). Prostaglandins are essential composed of two enzymes, cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). The expression of COX-2 is tightly regulated and induced through many inflammatory mediators such as cytokines, growth factors and endotoxin. Functionally, COX2 is a homodimer that is responsible for catalysing two steps during the conversion process of arachadonic acid into prostaglandin H2. It can be inducibly-expressed by many different cell types such as monocytes, endothelium, fibroblasts and vascular smooth muscle cells.

INTENDED USE

Rat COX-2 ELISA kit can be used for detecting quantitative levels of cyclooxygenase-2 (COX-2, COX2) in the lysates of tissues or cells. This assay has a minimum sensitivity detection limit of 0.16 ng/ml.

SENSITIVITY

The minimum sensitivity detection limit of cyclooxygenase-2 (COX-2, COX2) using this rat COX-2 ELISA kit was approximately 0.16 ng/ml. The dynamic assay range for this kit is 0.31 – 20.0 ng/ml.

REFERENCES

  1. BDNF and COX-2 participate in anti-depressive mechanisms of catalpol in rats undergoing chronic unpredictable mild stress. Physiol Behav. (2015) 151: 360-8. Wang J.M., et al.
  2. Differential expression of COX-2 in osteoarthritis and rheumatoid arthritis. Genet Mol Res. (2015) 14 (4): 12872-9. Fan H.W., et al.
  3. Does miRNA-155 Promote Cyclooxygenase-2 Expression in Cancer? Drug Dev Res. (2015) 76 (7): 354-6. Review. Comer B.S.
  4. Cyclooxygenase 2: its regulation, role and impact in airway inflammation. Clin Exp Allergy. (2016) 46 (3): 397-410. Review. Rumzhum N.N. and Ammit A.J.
  5. COX-2 inhibitors: a novel strategy in the management of breast cancer. Drug Discov Today. (2016) 21 (4): 598-615. Review. Regulski M., et al.

ADDITIONAL INFORMATION

  • Full Name: COX-2 ELISA Kit (Rat)
  • Reactivity: Rat
  • Sample Type: Tissue Lysates, Cell Lysates
  • Sensitivity: 0.16 ng/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford