Cortisol ELISA Kit

  • Created on the 2 February, 2017.


Cortisol (also called hydrocortisone and compound F) is a corticosteroid hormone or glucocorticoid that is produced in the zona fasciculate and the zona reticularis of the adrenal cortex. It is more commonly referred to as the “stress hormone” that is responsible for responding to stress. Over 90% of cortisol is found to be bound to cortisol-binding globulins (CBG) and around 7% is bound to albumin, only 1-3% is found unbound (this represent the active form of cortisol). Among the products that are released by the human adrenal cortex, only cortisol has been known to regulate ACTH secretion, for example the release of ACTH is inhibited through a negative feedback loop as the level of free cortisol (non-protein bound) in blood rises and the reverse happens when cortisol levels are subnormal.

The main biological effects of cortisol include deposition of liver glycogen, effect on fat metabolism, promotion of gluconeogenesis, anti-inflammatory action and the increase in blood glucose concentration when carbohydrate utilization has been reduced. Its measurement is a very useful indicator in diseases with abnormal glucocorticoid production e.g. Cushing Syndrome (hypercortisolism), Addison’s disease or even secondary adrenal insufficiency (hypocortisolism). Due to the diurnal fluctuation of cortisol levels it may be necessary to take several samples that are going to be used for an individual cortisol profile or used during dynamic tests such as ACTH stimulation and dexamethasone suppression tests.


Cortisol ELISA kit is a reliable quantitative procedure for measuring cortisol in human serum and plasma. This assay has a minimum sensitivity detection limit of 3.79 ng/ml.


The minimum sensitivity detection limit of cortisol using this human cortisol ELISA kit was approximately 3.79 ng/ml. The dynamic assay range for this kit is 10.0 – 800.0 ng/ml.


  1. Intranasal insulin attenuates the hypothalamic—pituitary—adrenal axis response to psychosocial stress. Psychoneuroendocrinology (2008) 33: 1394-1400. Andreas Bohringer A., et al.
  2. Thermoregulatory and endocrine responses to light pulses in short-day acclimated social voles (Microtus socialis).Chronobiology International (2007) 24 (2): 269-288. Zubidat AE, Ben-Shlomo R, Haim A.
  3. Effect of simultaneous versus apart administration of dexamethasone and alpha-ketoglutarate on growth hormone, cortisol and insulin-like growth factor-I in piglets. Bull Vet Inst Pulawy (2006) 50: 205-210. Sliwa E.


  • Full Name: Cortisol ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 3.79 ng/ml



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