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Corticosterone ELISA Kit (Rat/Mouse)

  • Created on the 27 October, 2017.

BACKGROUND

Corticosterone (also called 11β,21-dihydroxyprogesterone or 17-deoxycortisol) is produced in the cortex by the adrenal glands, a 21-carbon steroid hormone of the corticosteroid type. It is secreted by the adrenal cortex which is mainly controlled by the ACTH (a pituitary hormone) through a negative feedback mechanism. In rats, it represents one of the most abundant steroid that is circulating, due to the fact that rodents are unable to synthesize cortisol (a major glucocorticoid in human), this is because of a lack of the enzyme C17-Hydroxylase. The levels of corticosterone in nocturnal animals (such as in rats) display a distinct circadian variation with peak values in the latter portion of the day, that is followed by a nadir in the morning and this is thought to play an vital role in sleep-wake cyclus. Corticosterone is important for a large number of activities in rodents. It is vital in regulating protein, carbohydrate and fat metabolism. It is also responsible for influencing the hemopoietic system and can lead to reducing the total number of eosinophils and lymphocytes, but this is to a lesser extent when compared to cortisol. The measurement of corticosterone concentrations in rats is of particular interest to facilities that are conducting neurophysiological research and drug research departments.

INTENDED USE

Rat/mouse corticosterone ELISA kit is intended for detecting quantitative amounts of corticosterone in rat or mouse in samples of serum and plasma. This assay has a minimum sensitivity detection limit of 6.1 ng/ml.

SENSITIVITY

The minimum sensitivity detection limit of corticosterone using this rat/mouse corticosterone ELISA kit was approximately 6.1 ng/ml. The dynamic assay range for this kit is 15.0 – 2,250.0 ng/ml.

REFERENCES

  1. Development of an antigen heterologous enzyme linked immunosorbent assay for measurement of corticosterone in rat serum. J Immunoassay Immunochem. (2011) 32 (3): 244-57. Shrivastav T.G., et al.
  2. Effects of different timing of stress on corticosterone, BDNF and memory in male rats. Physiol Behav. (2015) 139: 459-67. Radahmadi M., et al.
  3. High plasma corticosterone levels persist during frequent automatic blood sampling in rats. In Vivo. (2005) 19 (5): 815-9. Abelson K.S., et al.
  4. Development of an enzyme-linked immunosorbent assay for serum 11-dehydrocorticosterone in rat and mouse. Biol Pharm Bull. (2007) 30 (3): 403-9. Tagawa N., et al.

ADDITIONAL INFORMATION

  • Full Name: Corticosterone ELISA Kit (Rat/Mouse)
  • Reactivity: Rat, Mouse
  • Sample Type: Plasma, Serum
  • Sensitivity: 6.1 ng/ml

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