Chromogranin A ELISA Kit (ChgA)

INTENDED USE

Human chromogranin A ELISA kit can be used for specifically detecting in vitro quantitative concentrations of chromogranin A (ChgA, parathyroid secretory protein 1) human serum and plasma. This assay has a minimum sensitivity detection limit of 1.4 ng/ml.

BACKGROUND

Chromogranin A (ChgA) is also called parathyroid secretory protein 1, consists of 439 amino acids that is a 49 kDa acidic protein encoded on chromosome 14. It belongs to the member of granin family of neuroendocrine secretory proteins and has been identified in a large number of normal and neoplastic endocrine tissues. Examples of cells that are producing this protein include; chromaffin cells of the medulla, adrenal, enterochromaffin-like, paraganglia and beta cells of the pancreas. They are present in the islet beta cell secretory granules.

There have been evidence to suggest that elevated circulating chromogranin A levels would be vital markers for tumors of neuroendocrine origin. However, recently, one of the most significant clinical use has been related to the diagnostic procedure in patients of pheochromocytoma. It is a precursor to several functional peptides such as pancreastatin, vasostatin-1, vasostatin-2, parastatin and catestatin, all of these peptides have the ability to negatively modulate the neuroendocrine function nearby cells (paracrine response) or releasing cell (autocrine response). It has the ability to induce and promote the generation of secretory granules such as those containing insulin within pancreatic islet beta cells.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Plate – Coated with human chromogranin A antibody.
• Standard A-E (Lyophilized) – Concentrations 0, 50, 150, 500, 1500 µg/l.
• Control 1 And Control 2 (Lyophilized).
• Conjugate.
• Biotin-Antibody.
• Adjustment Buffer.
• Wash Buffer Concentrated.
• Substrate Solution – Tetramethylbenzidine (TMB).
• Stop Solution.

SENSITIVITY

The minimum sensitivity detection limit of ChgA (chromogranin A, parathyroid secretory protein 1) using current chromogranin A ELISA kit was approximately 1.4 ng/ml. The dynamic range for this assay is 0 – 2,500 ng/ml.

ASSAY CHARACTERISTICS

– Expected Reference Values: < 100 µg/l
– Analytical Sensitivity (Limit of Detection): 10 µg/l.
– Linearity: r = 0985.
– Recovery: 106.1 %.
– Precision Intra-Assay: 8.9 %.
– Precision Inter-Assay: 9.5 %
– High-Dose Hook Effect: No high-dose hook effect up to 2 000 000 µg/l.

REFERENCES

1. A Critical Approach to Clinical Biochemistry of Chromogranin A. Adv Exp Med Biol. (2015) 867: 317-23. Review. Bottoni P., et al.
2. Chromogranin A: any relevance in neuroendocrine tumors? Curr Opin Endocrinol Diabetes Obes. (2016) 23 (1): 28-37. Review. Kidd M., et al.
3. The chromogranin A-derived peptides vasostatin-I and catestatin as regulatory peptides for cardiovascular functions. Cardiovasc Res. (2010) 85 (1): 9-16. Review. Helle K.B.
4. New biological aspects of chromogranin A-derived peptides: focus on vasostatins. Comp Biochem Physiol A Mol Integr Physiol. (2007) 147 (1): 11-8. Review. Tota B., et al.

ADDITIONAL INFORMATION

• Full Name: Chromogranin A ELISA Kit (ChgA)
• Reactivity: Human
• Sample Type: Serum, Plasma
• Sensitivity:  1.4 ng/ml

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