Human Chlamydia trachomatis IgG ELISA kit is designed for analysing in-vitro quantitative concentrations of human IgG antibodies against Chlamydia trachomatis (Chlamydia trachomatis-IgG) in serum and plasma samples. This assay has a minimum analytical sensitivity limit to a diagn. >95.0%.
Chlamydia trachomatis has been described as one of the most common bacterial sexually transmitted infections worldwide and also women are regarded as carrying the major burden of the disease. Chlamydial infection has been found to result in casing a cause disease in a number of different organ systems, which includes the genitourinary tract. C. trachomatis is a strong immunogen, which can stimulate both cell mediated and humoral immune responses. The general outcome of the chlamydial infection is largely dependent on the overall balance and interaction of cytokines that have been secreted by the activated lymphocytes. IFN-γ is regarded as an important factor which is vital in the host defence against Chlamydia, while on the other hand the susceptibility to the disease has been associated with enhanced expression of IL-10. The means that the changes to the immune system or even any disturbances which have been induced by C. trachomatis could either favour its own survival in the infected host or it could induce persistent infections.
CHLAMYDIA TRACHOMATIS IGG ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Cut-off Control.
- Positive Control.
- Negative Control.
- Chlamydia trachomatis anti-IgG Conjugate.
- IgG Sample Diluent.
- TMB Substrate Solution.
- Washing Buffer (20x Conc.).
- Stop Solution.
- Chlamydia trachomatis Coated Microplate (IgG).
The minimum detection sensitivity level of IgG antibodies against Chlamydia trachomatis using current human Chlamydia trachomatis-IgG ELISA kit was to a diagn. >95.0%. The dynamic range for this assay is to a specific cut-off point.
– Chlamydia Trachomatis ELISA: IgG
– Equivocal: 9 – 11 U
– Positive: > 11 U
– Cut-Off: 10 U
– Negative: < 9 U
– Diagnostic Specificity: 95.93%
– Diagnostic Sensitivity: 97.33%
– Cross Reactivity: No false positives identified.
– Interferences: No interferences using lipemic, haemolytic and icteric samples.
- Elevated serum Chlamydia trachomatis IgG antibodies. What do they mean for IVF pregnancy rates and loss? J Reprod Med. (1997) 42 (5): 281-6. Sharara F.I., et al.
- Chlamydia trachomatis IgG seropositivity is associated with lower natural conception rates in ovulatory subfertile women without visible tubal pathology. Hum Reprod. (2011) 26 (11): 3061-7. Coppus S.F., et al.
- [Chlamydia trachomatis antigens in endocervical samples and serum IgG antibodies in sterile-infertile women using ELISA]. Mikrobiyol Bul. (1992) 26 (3): 203-13. Turkish. Cengiz L., et al.
- The value of Chlamydia trachomatis-specific IgG antibody testing and hysterosalpingography for predicting tubal pathology and occurrence of pregnancy. Fertil Steril. (2007) 88 (1): 224-6. Perquin D.A., et al.
- Usefulness of enzyme linked immunosorbent assays species specific in the detection of Chlamydia trachomatis and Chlamydophila pneumoniae IgG antibodies in patients with genital infections or respiratory tract infections. Pathol Biol (Paris). (2008) 56 (3): 143-7. Frikha-Gargouri O., et al.
- Full Name: Chlamydia Trachomatis IgG ELISA Kit
- Reactivity: Human
- Sample Type: Plasma, Serum
- Sensitivity: diagn. >95.0%