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Chlamydia Pneumoniae IgG ELISA Kit

INTENDED USE

Human Chlamydia pneumoniae IgG ELISA kit is a procedure intended for in-vitro quantitative detection of human IgG class antibodies to Chlamydia pneumoniae (Chlamydia pneumoniae-IgG) in serum or plasma samples. This assay has a minimum analytical sensitivity limit to a diagn. 90.2%.

BACKGROUND

Chlamydia pneumoniae is a type of gram negative bacterium which can infect the lungs, the infection is characterised by a gradual onset of the symptoms that is followed by a long incubation period (3-4 weeks). C. pneumoniae can also infect many other human cells such as lymphocytes, macrophages, monocytes, smooth muscle, epithelial and endothelial cells. Also, pneumonia which has been caused by C. pneumoniae is usually regarded as atypical, since antibiotics which are used to treat pneumonia that has been caused by other types of bacteria do not work against Chlamydia pneumoniae infections. It has also be called an energy parasite because it requires a host cell for obtaining ATP and other high-energy intermediates. There is evidence to indicate that if chlamydial infections are untreated then this could result in chronic salpingitis, which could lead to infertility or even ectopic pregnancy. This most widely used procedures for detecting this pathogen or infection is by microscopy, PCR, antigen and antibody detection using ELISA methods.

CHLAMYDIA PNEUMONIAE IGG ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Chlamydia pneumoniae Coated Microplate (IgG).
  • Cut-off Control.
  • Positive Control.
  • Negative Control.
  • Chlamydia pneumoniae anti-IgG Conjugate.
  • TMB Substrate Solution.
  • IgG Sample Diluent.
  • Washing Buffer (20x Conc.).
  • Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of IgG class antibodies to Chlamydia pneumoniae using current human Chlamydia pneumoniae-IgG ELISA kit was to a diagn. 90.2%. The dynamic range for this assay is to a specific cut-off point.

ASSAY CHARACTERSTICS

– Chlamydia Pneumoniae ELISA: IgG
– Positive: > 11 U
– Negative: < 9 U
– Equivocal: 9 – 11 U
– Cut-Off: 10 U
– Diagnostic Specificity: 95.12%
– Diagnostic Sensitivity: 81.61%
– Cross Reactivity: No false-positive results were revealed.
– Interferences: Interferences with hemolytic, lipemic or icteric samples are not observed up to a concentration of 0.5 mg/ml bilirubin, 5 mg/ml triglycerides and 10 mg/ml hemoglobin.

REFERENCES

  1. The proatherogenic properties of lipoprotein(a) may be enhanced through the formation of circulating immune complexes containing Chlamydia pneumoniae-specific IgG antibodies. Eur Heart J. (2000) 21 (8): 639-46. Glader C.A., et al.
  2. The species specificity of the microimmunofluorescence antibody test and comparisons with a time resolved fluoroscopic immunoassay for measuring IgG antibodies against Chlamydia pneumoniae. J Clin Pathol. (1999) 52 (2): 99-102. Wong Y.K., et al.
  3. Chlamydia pneumoniae IgG seropositivity in deep-vein thrombosis. Lancet. (2000) 356 (9241): 1606-7. Maraha B., et al.
  4. Role of IgG-seropositivity to Chlamydia pneumoniae in early thrombotic events after coronary stent placement. Atherosclerosis. (2003) 166 (1): 171-6. da Costa C.P., et al.
  5. Detection of serum antibodies against Chlamydia pneumoniae by ELISA. FEMS Immunol Med Microbiol. (1996) 14 (2-3): 179-83. Numazaki K., et al.

ADDITIONAL INFORMATION

  • Full Name: Chlamydia Pneumoniae IgG ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: diagn. 90.2%

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