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Chikungunya Virus IgM µ-Capture ELISA Kit

INTENDED USE

Human Chikungunya virus IgM ELISA kit is intended for measuring in-vitro quantitative levels of human IgM antibodies to Chikungunya virus (Chikungunya IgM) using serum and plasma. This assay has a minimum analytical sensitivity limit to a diagn. >90%.

BACKGROUND

Chikungunya is a member of the Togaviridae family of the alphavirus genus, it can be described as a mosquito-borne viral disease. Some of the many symptoms of this disease include muscle pain, severe joint pain, headache, rash, fever and fatigue. Chikungunya is found all around the world and it shares a number of clinical signs which are similar to dengue and zika virus. The virus is transmitted by a bite from an infected female mosquitoes from one human to another. ELISA assays are one of the most used serological tests which are routinely utilised in order to diagnose Chikungunya virus. Usually either IgM or IgG anti-chikungunya antibodies are used and in the case for IgM, it has been discovered that they are at their highest levels around the 3-5 week period after the onset of the infection and this usually persists for another 2 months.

CHIKUNGUNYA IGM ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Chikungunya Virus Coated Microplate (IgM).
  • Sample Diluent.
  • Washing Buffer (20x Conc.).
  • Chikungunya Virus Antigen (Lyophilized).
  • Biotinylated Chikungunya Virus Antibody.
  • Streptavidin Conjugate.
  • TMB Substrate Solution.
  • Positive Control.
  • Cut-Off Control.
  • Negative Control.

SENSITIVITY

The minimum detection sensitivity level of IgM antibodies to Chikungunya virus (Chikungunya-IgM) using current human Chikungunya IgM ELISA kit was to a diagn. >90%. The dynamic range for this assay is to a specific cut-off point.

ASSAY CHARACTERSTICS

– Chikungunya Virus ELISA: IgM
– Substrate Blank: Absorbance value < 0.100
– Negative Control: Absorbance value < Cut-Off
– Cut-off Control: Absorbance value 0.150 – 1.300
– Positive Control: Absorbance value > Cut-Off
– Interferences: Interferences with hemolytic, lipemic or icteric samples are not observed up to a concentration of 10 mg/mL hemoglobin, 5 mg/mL triglycerides and 0.5 mg/mL bilirubin.
– Cross Reactivity: Cross reactivity with antibodies against Borrelia, CMV and Toxoplasma cannot be excluded. Interference with polyclonal stimulation of EBV infections is likely.

REFERENCES

  1. Evaluation of two IgM rapid immunochromatographic tests during circulation of Asian lineage Chikungunya virus. Southeast Asian J Trop Med Public Health. (2012) 43 (1): 55-61. Kosasih H., et al.
  2. Development and Evaluation of an in-House IgM-Capture ELISA for the Detection of Chikungunya and Its Application to a Dengue Outbreak Situation in Kenya in 2013. Jpn J Infect Dis. (2015) 68 (5): 410-4. Wasonga C., et al.
  3. Cloning, expression and evaluation of diagnostic potential of recombinant capsid protein based IgM ELISA for chikungunya virus. J Virol Methods. (2014) 203: 15-22. Priya R., et al.
  4. Correlation of plasma viral loads and presence of Chikungunya IgM antibodies with cytokine/chemokine levels during acute Chikungunya virus infection. J Med Virol. (2014) 86 (8): 1393-401. Reddy V., et al.
  5. [Dengue, Zika and Chikungunya]. Medicina (B Aires). (2016) 76 (2): 93-7. Spanish. Kantor I.N.
  6. A potent neutralizing IgM mAb targeting the N218 epitope on E2 protein protects against Chikungunya virus pathogenesis. MAbs. (2015) 7 (6): 1178-94. Lam S., et al.

ADDITIONAL INFORMATION

  • Full Name: Chikungunya Virus IgM µ-Capture ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: daign. >90%

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