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Chikungunya Virus IgM µ-Capture ELISA Kit

  • Created on the 13 July, 2017.

INTENDED USE

Human Chikungunya virus IgM µ-capture ELISA kit is intended for measuring in-vitro quantitative levels of human IgM antibodies to Chikungunya virus using serum and plasma. This assay has a minimum analytical sensitivity limit to a diagn. >90%.

BACKGROUND

Chikungunya is a member of the Togaviridae family of the alphavirus genus, it can be described as a mosquito-borne viral disease. Some of the many symptoms of this disease include muscle pain, severe joint pain, headache, rash, fever and fatigue. Chikungunya is found all around the world and it shares a number of clinical signs which are similar to dengue and zika virus. The virus is transmitted by a bite from an infected female mosquitoes from one human to another. ELISA assays are one of the most used serological tests which are routinely utilised in order to diagnose Chikungunya virus. Usually either IgM or IgG anti-chikungunya antibodies are used and in the case for IgM, it has been discovered that they are at their highest levels around the 3-5 week period after the onset of the infection and this usually persists for another 2 months.

SENSITIVITY

The minimum detection sensitivity level of IgM antibodies to Chikungunya virus using current human Chikungunya virus IgM µ-capture ELISA kit was to a diagn. >90%. The dynamic range for this assay is to a specific cut-off point.

REFERENCES

  1. Evaluation of two IgM rapid immunochromatographic tests during circulation of Asian lineage Chikungunya virus. Southeast Asian J Trop Med Public Health. (2012) 43 (1): 55-61. Kosasih H., et al.
  2. Development and Evaluation of an in-House IgM-Capture ELISA for the Detection of Chikungunya and Its Application to a Dengue Outbreak Situation in Kenya in 2013. Jpn J Infect Dis. (2015) 68 (5): 410-4. Wasonga C., et al.
  3. Cloning, expression and evaluation of diagnostic potential of recombinant capsid protein based IgM ELISA for chikungunya virus. J Virol Methods. (2014) 203: 15-22. Priya R., et al.
  4. Correlation of plasma viral loads and presence of Chikungunya IgM antibodies with cytokine/chemokine levels during acute Chikungunya virus infection. J Med Virol. (2014) 86 (8): 1393-401. Reddy V., et al.
  5. [Dengue, Zika and Chikungunya]. Medicina (B Aires). (2016) 76 (2): 93-7. Spanish. Kantor I.N.
  6. A potent neutralizing IgM mAb targeting the N218 epitope on E2 protein protects against Chikungunya virus pathogenesis. MAbs. (2015) 7 (6): 1178-94. Lam S., et al.

ADDITIONAL INFORMATION

  • Full Name: Chikungunya Virus IgM µ-Capture ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: daign. >90%

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