CDV IgG ELISA Kit (Canine Distemper Virus)

Full Name: CDV IgG ELISA Kit (Canine Distemper Virus)
Reactivity: Canine/Dog
Sample Type: Plasma, Serum
Sensitivity: Qualitative

BACKGROUND

Canine distemper virus affects animal families that includes; dogs, wolves, foxes, coyotes, raccoons, ferrets and skunks. In canines, it is found to impact respiratory and gastrointestinal tracts, the brain and the spinal cord. CDV can be caused by a single-stranded RNA virus that belong to the Paramyxoviridae family, it is also closely related to the viruses that result in rinderpest and measles. Distemper virus causes a wide range of clinical signs and symptoms in canines. These include, anorexia, ocular discharge, dermatitis (urticae), conjunctivitis, erythema and lymphadenopathy. Infection has been associated with pulmonary interstitial pneumonia in dogs and other canines. Affected animals may experience secondary infections such as bacteremia or pyothorax. In the late stages of disease when distemper virus is replicating in the lungs, there may be extensive necrosis of lung tissue with fluid accumulation. There have been reports of distemper being detected during necropsies performed on horses that died suddenly.

Clinical signs of CDV are variable and include respiratory distress, congestion, edema and pulmonary edema. On the right side of the thorax or on one or both sides of the neck, lung lesions were frequently discovered. In some instances a left lateral cervical lymph node enlargement was noted at necropsy with no evidence of a cause other than possible a distemper viral infection as determined through serologic investigations. In order to prevent canine distemper, puppies need to be vaccinated at 6-8 weeks of age and then continue to have a “booster shot” every 2-4 weeks until reaching 16 weeks of age. The whole course of vaccination program needs to be undertaken, otherwise the vaccination will not provide any protection from the virus. Canine distemper is a highly infectious and deadly virus that affects various species.

INTENDED USE

CDV IgG ELISA kit is a method for analysing IgG antibodies against canine distemper virus (CDV) in plasma or serum.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Strips Coated With CDV Proteins.
CDV Negative Control.
CDV Positive Control.
Strip Holder.
HRPO-Conjugated (IgG)(Anti-Species) Antibody.
ELISA Buffer.
Wash Solution (200x Concentrated).
Substrate Buffer A.
Substrate Buffer B.
Plastic Cover Seal.
Stop Solution.

SENSITIVITY

The standard range of IgG antibodies against canine distemper virus (CDV) using current CDV IgG ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Quantitatively (End Point Titer):
(i). < 30: No antibodies found.
(ii). 90 – 270: Antibodies found, probably shedding CCV, retest in 3 months.
(iii). > 810: High titer of antibodies found in recovered diseased animals. Diseased animal (suggestive for CDV). Healthy animal (retest in 3 months).

REFERENCES

Antigen requirements and specificity of enzyme-linked immunosorbent assay for detection of canine IgG against canine distemper viral antigens. Am J Vet Res. (1982) 43 (12): 2266-9. Bernard S.L., et al.
Accuracy of a point-of-care ELISA test kit for predicting the presence of protective canine parvovirus and canine distemper virus antibody concentrations in dogs. Vet J. (2012) 193 (2): 363-6. Litster A.L., et al.
Virological and serological findings in dogs with naturally occurring distemper. J Virol Methods. (2015) 213: 127-30. Elia G., et al.
Serological analysis of canine distemper virus using an immunocapture ELISA. J Vet Med Sci. (1996) 58 (8): 791-4. Gemma T., et al.

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