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CCV (Canine Coronavirus) IgM ELISA Kit (Dog)

INTENDED USE

Canine coronavirus IgM ELISA kit can be used for analysing IgM antibodies against canine coronavirus (CCV)(dog) in blood and plasma samples. This assay has a quantitative and qualitative standard range.

BACKGROUND

Canine Coronavirus (CCV) can cause intestinal disease in dogs and is a member of the coronaviridae family. In most cases the infected dogs experience a complete recover and develop immunity to CCV. The virus invades and replicates in the villi of the small intestine, in majority of instances this can also make the cells more susceptible to infection by parvoviruses. Looking at clinical history, clinical sign and performing antigen detection are often carried out in the process of diagnosing CCV. Following procedure is designed to determine corona virus antibodies in blood or plasma samples, it is also well known that majority of positive dogs are potential virus carriers and therefore will shed CCV.

CANINE CORONAVIRUS IGM ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Strips Coated With CCV Proteins.
  • Weak Positive Control.
  • Negative Control.
  • Strip Holder.
  • HRPO-Conjugated (IgM)(Anti-Species) Antibody.
  • Wash Solution (200x Concentrated).
  • ELISA Buffer.
  • Substrate Buffer A.
  • Substrate Buffer B.
  • Stop Solution.
  • Plastic Cover Seal.

SENSITIVITY

The standard range of IgM antibodies to CCV using current canine coronavirus (CCV) IgM ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Canine Coronavirus (CAV) IgM: ELISA
– Qualitatively: Sample is scored positive if the OD is higher than 2.5 x OD of the negative control.
– Quantitatively (End Point Titer):
< 30: No antibodies found.
90 – 270: Antibodies found, probably shedding CCV, retest in 3 months.
> 810: High titer of antibodies found in recovered diseased animals.

REFERENCES

  1. Isolation, tissue distribution and molecular characterization of two recombinant canine coronavirus strains. Vet Microbiol. (2011) 151 (3-4): 238-44. Ntafis V., et al.
  2. Sequence analysis of divergent canine coronavirus strains present in a UK dog population. Virus Res. (2009) 141 (1): 21-5. Erles K. and Brownlie J.
  3. Antibody testing against canine coronavirus by immunoperoxidase plaque staining. Vet Res Commun. (2001) 25 (4): 327-36. Soma T., et al.
  4. Canine coronavirus highly pathogenic for dogs. Emerg Infect Dis. (2006) 12 (3): 492-4. Buonavoglia C., et al.

ADDITIONAL INFORMATION

  • Full Name: CCV (Canine Coronavirus) IgM ELISA Kit (Dog)
  • Reactivity: Dog
  • Sample Type: Plasma, Serum
  • Sensitivity:  Quantitative, Qualitative

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