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CCV (Canine Coronavirus) IgG ELISA Kit (Dog)

INTENDED USE

Canine coronavirus IgG ELISA kit is designed to determine IgG antibodies against canine coronavirus (CCV)(dog) in samples of serum and plasma. This assay has a quantitative and qualitative standard range.

BACKGROUND

Canine Coronavirus (CCV) belongs to the coronaviridae family and is responsible for causing an intestinal disease in both wild and domestic dogs. However, the majority of infected dogs usually completely recover and also develop immunity against CCV. The dogs which are unable to recover and not build immunity to CCV, results in the disease progressing into a fatal form. This fatal form is a chronic and progressive disease which can lead to weakness, loss of appetite, diarrhea and many intestinal diseases. The virus is able to invade and replicate in the small intestine (specifically in the villi), this can result in the cells becoming more susceptible to parvovirus infection. It is important during the diagnosis of CCV to look at clinical history, clinical sign and carry out antigen detection procedures. The majority of antibody positive dogs tend to be virus carriers and therefore will most likely shed CCV.

CANINE CORONAVIRUS IGG ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Strips Coated With Canine Coronavirus Proteins.
  • Strip Holder.
  • HRPO-Conjugated (IgG)(Anti-Species) Antibody.
  • CCV Weak Positive Control.
  • CCV Negative Control.
  • Wash Solution (200x Concentrated).
  • ELISA Buffer.
  • Substrate Buffer A.
  • Substrate Buffer B.
  • Stop Solution.
  • Plastic Cover Seal.

SENSITIVITY

The standard range of IgG antibodies to CCV using current canine coronavirus (CCV) IgG ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Canine Coronavirus (CAV) IgG: ELISA
– Qualitatively (Positive or Negative): Sample is scored positive if the OD is higher than 2.5 x OD of the negative control.
– Quantitatively (End Point Titer):
< 30 = no antibodies found.
90 – 270: Antibodies found, probably shedding CCV, retest in 3 months.
> 810: High titer of antibodies found in recovered diseased animals.

REFERENCES

  1. Effect of recombinant lactobacillus expressing canine GM-CSF on immune function in dogs. J Microbiol Biotechnol. (2009) 19 (11): 1401-7. Chung J.Y., et al.
  2. Canine coronavirus infection in the dog following oronasal inoculation. Res Vet Sci. (1991) 51 (1): 11-8. Tennant B.J., et al.
  3. Prolonged depletion of circulating CD4+ T lymphocytes and acute monocytosis after pantropic canine coronavirus infection in dogs. Virus Res. (2010) 152 (1-2): 73-8. Marinaro M., et al.
  4. Development and evaluation of an ELISA to measure antibody responses to both the nucleocapsid and spike proteins of canine coronavirus. J Immunoassay. (1998) 19 (1): 1-22. Palmer-Densmore M.L., et al.

ADDITIONAL INFORMATION

  • Full Name: CCV (Canine Coronavirus) IgG ELISA Kit (Dog)
  • Reactivity: Dog
  • Sample Type: Serum, Plasma
  • Sensitivity:  Qualitative, Quantitative

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