CCV IgG ELISA Kit (Canine Coronavirus)

Full Name: CCV IgG ELISA Kit (Canine Coronavirus)
Reactivity: Canine/Dog
Sample Type: Serum, Plasma
Sensitivity: Qualitative

BACKGROUND

Canine Coronavirus (CCV) belongs to the coronaviridae family and can result in an intestinal disease for both wild and domestic dogs. However, the majority of infected dogs usually completely recover and also develop immunity against the virus. The dogs which are unable to recover and not build immunity to CCV, results in the disease progressing into a fatal form. This fatal form is a chronic and progressive disease which can lead to weakness, loss of appetite, diarrhea and many intestinal diseases. The virus can also enter into a new host if a contaminated object is introduced into their environment. It had been noted that dogs under 2 years of age are most susceptible to getting this lethal variant of CCV in the majority of cases. However, some sources of evidence have told us that the age of vulnerability to CCV is 2–5 years old. This suggests that puppies under the age of two may not be as susceptible to contracting this lethal version of the illness.

The villi of the small intestine are where the virus can enter and reproduce, making the cells there more vulnerable to parvovirus infection. It is important during the diagnosis process to look at clinical history, clinical sign and carry out antigen detection procedures. The majority of antibody positive dogs tend to be virus carriers and therefore will most likely shed the virus.

INTENDED USE

CCV IgG ELISA kit is designed to determine IgG antibodies against canine coronavirus (CCV) in samples of serum and plasma.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Strips Coated With Canine Coronavirus Proteins.
Strip Holder.
HRPO-Conjugated (IgG)(Anti-Species) Antibody.
CCV Weak Positive Control.
CCV Negative Control.
Wash Solution (200x Concentrated).
ELISA Buffer.
Substrate Buffer A.
Substrate Buffer B.
Stop Solution.
Plastic Cover Seal.

SENSITIVITY

The standard range of IgG antibodies to canine coronavirus (CCV) using current CCV IgG ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Qualitatively (Positive or Negative): Sample is scored positive if the OD is higher than 2.5 x OD of the negative control.
– Quantitatively (End Point Titer):
< 30 = no antibodies found.
90 – 270: Antibodies found, probably shedding CCV, retest in 3 months.
> 810: High titer of antibodies found in recovered diseased animals.

REFERENCES

Effect of recombinant lactobacillus expressing canine GM-CSF on immune function in dogs. J Microbiol Biotechnol. (2009) 19 (11): 1401-7. Chung J.Y., et al.
Canine coronavirus infection in the dog following oronasal inoculation. Res Vet Sci. (1991) 51 (1): 11-8. Tennant B.J., et al.
Prolonged depletion of circulating CD4+ T lymphocytes and acute monocytosis after pantropic canine coronavirus infection in dogs. Virus Res. (2010) 152 (1-2): 73-8. Marinaro M., et al.
Development and evaluation of an ELISA to measure antibody responses to both the nucleocapsid and spike proteins of canine coronavirus. J Immunoassay. (1998) 19 (1): 1-22. Palmer-Densmore M.L., et al.

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