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CCL11 (Eotaxin) ELISA Kit

  • Created on the 17 March, 2017.

BACKGROUND

C-C motif chemokine 11 (CCL11) which is also commonly referred to as eosinophil chemotactic protein and eotaxin-1, is a potent inducer of eosinophil chemotaxis and a cytokine belonging to the CC chemokine family. It is expressed on Th2 lymphocytes, eosinophils and basophils, also considered as a popular selective ligand of the CC chemokine receptor 3 (CCR3). CCL11 gene is located on chromosome 17 and is encoded on three exons.

Eotaxin is thought to be involved in eosinophilic inflammatory diseases such as allergic rhinitis, atopic dermatitis, parasitic infections and asthma. It is expressed constitutively at high levels in the colon and small intestine, the effects of CCL11 are found to be mediated through its binding to a G-protein-linked receptor that is known as a chemokine receptor. The chemokine receptors for which CCL11 is a potential ligand include CCR2, CCR3 and also CCR5. It has been found that eotaxin-1 has a high degree selectivity for its receptor, to the extent that these are inactive on monocytes and neutrophils, both of which do not express CCR3. The human eotaxin gene has been cloned and it displays approx. 61.8 and 63.2% similar amino acid sequence to guinea pig and mouse eotaxin. Increased levels of CCL11 within the blood plasma have been associated with aging in mice and humans.

INTENDED USE

CCL11 ELISA kit can be used for analysing in vitro quantitative levels of CCL11 (eotaxin) in human cell culture supernatant, serum and plasma (citrate, EDTA). This assay has a minimum detection limit of < 2.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of CCL11 (eotaxin) using this human C1CL11 ELISA kit was approximately 2.0 pg/ml. The dynamic assay range for this kit is 15.6 – 1,000 pg/ml.

REFERENCES

  1. From airway inflammation to inflammatory bowel disease: eotaxin-1, a key regulator of intestinal inflammation. Clin Immunol. (2014) 153 (1): 199-208. Review. Adar T., et al.
  2. CCL11 as a potential diagnostic marker for asthma? J Asthma. (2014) 51 (8): 847-54. Review. Wu D., et al.
  3. An Overlook to the Characteristics and Roles Played by Eotaxin Network in the Pathophysiology of Food Allergies: Allergic Asthma and Atopic Dermatitis. Inflammation. (2016) 39 (3): 1253-67. Review. Ahmadi Z., et al.
  4. New chemokine targets for asthma therapy. Curr Allergy Asthma Rep. (2005) 5 (2): 155-60. Review. Garcia G., et al.
  5. Eotaxin: from an eosinophilic chemokine to a major regulator of allergic reactions. Immunol Today. (1999) 20 (11): 500-4. Review. Gutierrez-Ramos J.C., et al.

ADDITIONAL INFORMATION

  • Full Name: CCL11 (Eotaxin) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum , Plasma (Citrate, EDTA), Cell Culture Supernatant
  • Sensitivity: < 2.0 pg/ml

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