CCL11 ELISA kit can be used for analysing in vitro quantitative levels of CCL11 (eotaxin) in human cell culture supernatant, serum and plasma (citrate, EDTA). This assay has a minimum detection limit of < 2.0 pg/ml.
C-C motif chemokine 11 (CCL11) which is also commonly referred to as eosinophil chemotactic protein and eotaxin-1, is a potent inducer of eosinophil chemotaxis and a cytokine belonging to the CC chemokine family. It is expressed on Th2 lymphocytes, eosinophils and basophils, also considered as a popular selective ligand of the CC chemokine receptor 3 (CCR3). CCL11 gene is located on chromosome 17 and is encoded on three exons.
The following CCL11 ELISA kit is designed to be used for analysing various diagnostic applications. Eotaxin is thought to be involved in eosinophilic inflammatory diseases such as allergic rhinitis, atopic dermatitis, parasitic infections and asthma. It is expressed constitutively at high levels in the colon and small intestine, the effects of CCL11 are found to be mediated through its binding to a G-protein-linked receptor that is known as a chemokine receptor. The chemokine receptors for which CCL11 is a potential ligand include CCR2, CCR3 and also CCR5. It has been found that eotaxin-1 has a high degree selectivity for its receptor, to the extent that these are inactive on monocytes and neutrophils, both of which do not express CCR3. The human eotaxin gene has been cloned and it displays approx. 61.8 and 63.2% similar amino acid sequence to guinea pig and mouse eotaxin. Increased levels of CCL11 within the blood plasma have been associated with aging in mice and humans. This CCL11 ELISA kit offers a sensitivity limit of 2.0 pg/ml and can be used to measure human samples of serum, cell culture supernatant and plasma.
The minimum detection sensitivity level of eotaxin (CL11) using current human CL11 ELISA kit was approximately 2.0 pg/ml. The dynamic range for this assay is 15.6 – 1,000 pg/ml.
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- Full Name: CCL11 (Eotaxin) ELISA Kit
- Reactivity: Human
- Sample Type: Serum , Plasma (Citrate, EDTA), Cell Culture Supernatant
- Sensitivity: < 2.0 pg/ml