- Created on the 28 March, 2017.
Cathepsin D is an aspartic endo-protease which is found to be distributed ubiquitously in lysosomes, in humans it is encoded by the CTSD gene. The main functions of this protein include degrading proteins and activating precursors of bioactive proteins within pre-lysosomal compartments. This proteinase is a family member of the peptidase A1, it displays specificity which is very similar to but also narrower than that of pepsin A. The catalytic sites of cathepsin D have two critical aspartic residues (33 and 231) which are found to be located on the 14 kDa and 34kDa chains. These two chains are linked by hydrophobic bonds and the mature cathepsin D is made of 337 amino acid residues, with 196 amino acid residues representing the heavy chain and 141 the light chain.
The main physiological functions of cathepsin D include degradation of growth factors and polypeptide hormones, processing of enzyme inhibitors and activators, metabolic degradation of intracellular proteins, activation of enzymatic precursors, regulation of programmed cell death and brain antigen processing. Over-expression of cathepsin D is found to stimulate metastasis and tumorigenicity, there are also evidence for it initiating tumor apoptosis. Mutations in this gene are known to be involved in the pathogenesis of several diseases such as Alzheimer disease and breast cancer. This protease has been used as an independent marker of poor prognosis in breast cancer, a useful tool in correlating with the incidence of clinical metastasis.
Human cathepsin-D ELISA kit can be used to determine in vitro quantitative levels of cathepsin D in human plasma, cell culture supernatant and serum. This assay has a minimum detection limit of < 10.0 pg/ml.
The minimum detection sensitivity level of cathepsin D using this human cathepsin-D ELISA kit was 10.0 pg/ml. The dynamic assay range for this kit is 156.0 – 10,000 pg/ml.
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- Prognostic significance of the estrogen-regulated proteins, cathepsin-D and pS2, in breast cancer. Minerva Med. (1998) 89 (1-2): 5-10. Review. Fulco R.A., et al.
- Significance of the tumor protease cathepsin D for the biology of breast cancer. Histol Histopathol. (2014) 29 (4): 433-8. Review. Dian D., et al.
- The Role of Cathepsin D in the Pathogenesis of Human Neurodegenerative Disorders. Med Res Rev. (2016) 36 (5): 845-70. Review. Vidoni C., et al.
- Cathepsin D: newly discovered functions of a long-standing aspartic protease in cancer and apoptosis. Cancer Lett. (2006) 237 (2): 167-79. Review. Liaudet-Coopman E., et al.
- Full Name: Cathepsin D ELISA Kit
- Reactivity: Human
- Sample Type: Cell Culture Supernatant. Plasma, Serum
- Sensitivity: < 10.0 pg/ml