GET A QUOTE

Cathepsin D ELISA Kit

  • Created on the 28 March, 2017.

BACKGROUND

Cathepsin D is an aspartic endo-protease which is found to be distributed ubiquitously in lysosomes, in humans it is encoded by the CTSD gene. The main functions of this protein include degrading proteins and activating precursors of bioactive proteins within pre-lysosomal compartments. This proteinase is a family member of the peptidase A1, it displays specificity which is very similar to but also narrower than that of pepsin A. The catalytic sites of cathepsin D have two critical aspartic residues (33 and 231) which are found to be located on the 14 kDa and 34kDa chains. These two chains are linked by hydrophobic bonds and the mature cathepsin D is made of 337 amino acid residues, with 196 amino acid residues representing the heavy chain and 141 the light chain.

The main physiological functions of cathepsin D include degradation of growth factors and polypeptide hormones, processing of enzyme inhibitors and activators, metabolic degradation of intracellular proteins, activation of enzymatic precursors, regulation of programmed cell death and brain antigen processing. Over-expression of cathepsin D is found to stimulate metastasis and tumorigenicity, there are also evidence for it initiating tumor apoptosis. Mutations in this gene are known to be involved in the pathogenesis of several diseases such as Alzheimer disease and breast cancer. This protease has been used as an independent marker of poor prognosis in breast cancer, a useful tool in correlating with the incidence of clinical metastasis.

INTENDED USE

Human cathepsin-D ELISA kit can be used to determine in vitro quantitative levels of cathepsin D in human plasma, cell culture supernatant and serum. This assay has a minimum detection limit of < 10.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of cathepsin D using this human cathepsin-D ELISA kit was 10.0 pg/ml. The dynamic assay range for this kit is 156.0 – 10,000 pg/ml.

REFERENCES

  1. Cathepsin D–many functions of one aspartic protease. Crit Rev Oncol Hematol. (2008) 68 (1): 12-28. Review. Benes P., et al.
  2. Prognostic significance of the estrogen-regulated proteins, cathepsin-D and pS2, in breast cancer. Minerva Med. (1998) 89 (1-2): 5-10. Review. Fulco R.A., et al.
  3. Significance of the tumor protease cathepsin D for the biology of breast cancer. Histol Histopathol. (2014) 29 (4): 433-8. Review. Dian D., et al.
  4. The Role of Cathepsin D in the Pathogenesis of Human Neurodegenerative Disorders. Med Res Rev. (2016) 36 (5): 845-70. Review. Vidoni C., et al.
  5. Cathepsin D: newly discovered functions of a long-standing aspartic protease in cancer and apoptosis. Cancer Lett. (2006) 237 (2): 167-79. Review. Liaudet-Coopman E., et al.

ADDITIONAL INFORMATION

  • Full Name: Cathepsin D ELISA Kit
  • Reactivity: Human
  • Sample Type: Cell Culture Supernatant. Plasma, Serum
  • Sensitivity: < 10.0 pg/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford