GET A QUOTE

Caspase 8 (CASP8) ELISA Kit

  • Created on the 5 April, 2017.

BACKGROUND

Caspase 8 (CASP8) is a member of family of conserved cysteine-aspartic acid proteinases which have an important role in the regulation of apoptosis and cell death. This protein is able to induce Fas and a number of other apoptotic stimuli in order to carry out programmed cell death. The process of apoptosis is essentially induced via the tumor necrosis factor receptor 1 (TNFR1) and Fas/APO1 (CD95) receptor, this leads to the activation of caspase 8 which causes the release of the active fragments p10 and p18. The results in the activation of other downstream effector caspases (notably 1, 3, 6 and 7), it is caspase 3 which is responsible for eliciting the common morphological hallmarks of apoptosis such as cell shrinkage and DNA fragmentation.

There are evidence for many cancer therapies which are in development that involve using molecules that trigger the activation of Caspase-8 mediated apoptosis, especially in cancer cells. There are other therapies that use compounds which are able to disrupt cancer-specific mechanisms in order to inhibit caspase-8 mediated apoptosis. Due to the fact that the activation of caspase-8 occurring at a such early stage of the apoptosis pathway, the detection of caspase-8 provides a useful alternative to measurement of apoptosis using Annexin V-FITC / PI, that measure phosphatidylserine (PS) present on the surface of apoptotic cells.

INTENDED USE

Human caspase 8 ELISA kit is intended for determining in vitro quantitative amounts of caspase-8 (CASP8) in human tissue homogenates, cell culture supernatant, plasma, cell lysates, serum and many biological fluids. This assay has a minimum sensitivity limit of < 0.052 ng/ml.

SENSITIVITY

Minimum detection limits of a caspase-8 using this human caspase 8 ELISA kit was 0.052 ng/ml. The dynamic assay range for this kit is 0.1 – 30.0 ng/ml.

REFERENCES

  1. Evolutionary analyses of caspase-8 and its paralogs: Deep origins of the apoptotic signaling pathways. Bioessays. (2015) 37 (7): 767-76. Review. Sakamaki K., et al.
  2. Old, new and emerging functions of caspases. Cell Death Differ. (2015) 22 (4): 526-39. Review. Shalini S., et al.
  3. Caspase-8 in apoptosis: the beginning of “the end”? IUBMB Life. (2000) 50 (2): 85-90. Review. Kruidering M. and Evan G.I.
  4. Caspases Connect Cell-Death Signaling to Organismal Homeostasis. Immunity. (2016) 44 (2): 221-31. Review. Galluzzi L., et al.
  5. Caspase-8 as a therapeutic target in cancer. Cancer Lett. (2013) 332 (2): 133-40. Review. Stupack D.G.

ADDITIONAL INFORMATION

  • Full Name: Caspase 8 (CASP8) ELISA Kit
  • Reactivity: Human
  • Sample Type: Cell Culture Supernatant, Cell Lysates, Serum, Tissue Homogenates, Plasma, Other Biological Fluids
  • Sensitivity: < 0.052 ng/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford