- Created on the 5 April, 2017.
Caspase 2 (CASP2) is an enzyme which is a class I caspases, known to contain a long pro-domain in order for nuclear localisation and is produced as a zymogen. It belongs to the cysteine family of proteases which has the ability to cleave proteins at the amino acid which proceeds an aspartic acid residue. In nearly all mammals CASP2 orthologs have been discovered for which the full genome data is available. There are unique orthologs which can be present in lizards, teleosts, birds and lissamphibians.
Caspase 2 can carry out proteolytic cleavage of other proteins and it possesses a similar amino acid sequence to some of the initiator caspases such as caspase-1, caspase-4, caspase-5, and caspase-9. The sequential activation of caspases is important in the execution-phase of cell apoptosis. It exists in an inactive caspase pro-enzyme from which needs to undergo a proteolytic process that can be located to the conserved aspartic residues. This event leads to the generation of two subunits, large (p19) and small (p12), both of these are able to dimerise in order to form the active enzyme. There are a variety of apoptotic stimuli which induce the proteolytic cleavage of this protein. It is essentially a nuclear apoptotic respondent to mitotic catastrophe or cellular genotoxic stress. The activation of caspase 2 occurs following the recruitment to a complex containing a p53-induced death domain protein (PIDD). Overall, it appears that caspase 2 is a very versatile caspase that has multiple functions beyond cell death induction.
Human caspase 2 ELISA kit can be used for detecting in vitro quantitative levels of caspase-2 (CASP2) in human serum, cell culture supernatant, plasma, tissue homogenates, cell lysates and other biological fluids. This assay has a minimum sensitivity limit of < 0.121 ng/ml.
The minimum detection sensitivity level of a caspase-2 using this human caspase 2 ELISA kit was 0.121 ng/ml. The dynamic assay range for this kit is 0.313 – 20.0 ng/ml.
- The role of caspase-2 in stress-induced apoptosis. J Cell Mol Med. (2010) 14 (6A): 1212-24. Review. Bouchier-Hayes L.
- Properties of the caspases. Biochim Biophys Acta. (1998) 1387 (1-2): 17-31. Review. Stennicke H.R. and Salvesen G.S.
- Cell death by mitotic catastrophe: a molecular definition. Oncogene. (2004) 23 (16): 2825-37. Review. Castedo M., et al.
- Caspase 2 in apoptosis, the DNA damage response and tumour suppression: enigma no more? Nat Rev Cancer. (2009) 9 (12): 897-903. Review. Kumar S.
- P53-induced protein with a death domain (PIDD): master of puppets? Oncogene. (2012) 31 (45): 4733-9. Review. Bock F.J., et al.
- Full Name: Caspase 2 (CASP2) ELISA Kit
- Reactivity: Human
- Sample Type: Cell Culture Supernatant, Tissue Homogenates, Serum, Plasma, Biological Fluids, Cell Lysates
- Sensitivity: < 0.121 ng/ml