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Cashew ELISA Kit

  • Created on the 4 October, 2017.

BACKGROUND

Cashew tree (Anacardium occidentale) is a tropical evergreen tree that belongs to the Anacardiaceae family and it produces cashew seed and cashew apple. These seed shells have been used to yields derivatives which are useful in many applications such as paints, waterproofing and lubricants. Cashews have a lower fat content when compared to most other nuts, approx. 82% is unsaturated fatty acids and around 66% of this is heart-healthy monounsaturated fats. Studies have indicated that people allergic to cashew are also allergic to pistachio (process called cross-reactivity), both of these nuts are botanically related. Cashew food allergies are usually linked to the presence of IgE antibodies which are directed against the major seed storage proteins in Cashew (namely 13S globulin proteins and 2S albumins). It has been found to frequently induce symptoms of food allergy, hypersensitivity reactions and atopic dermatitis in individuals that are sensitised. The following cashew ELISA assay method is a highly sensitive detection system that is able to quantify cashew residues in cereals, cookies, chocolate and ice-cream.

INTENDED USE

Cashew ELISA kit is designed for determining quantitative levels of cashew in food (such as chocolate, ice-cream, cereals and cookies). This assay has a minimum analytical sensitivity limit of 0.2 ppm.

SENSITIVITY

The minimum detection sensitivity level of cashew using this cashew ELISA kit was 0.2 ppm. The standard assay range for this kit is 2.0 – 60.0 ppm.

REFERENCES

  1. Sandwich enzyme-linked immunosorbent assay (ELISA) for detection of cashew nut in foods. J Food Sci. (2011) 76 (9): T218-26. Gaskin F.E. and Taylor S.L.
  2. In vitro digestion of soluble cashew proteins and characterization of surviving IgE-reactive peptides. Mol Nutr Food Res. (2014) 58 (4): 884-93. Mattison C.P., et al.
  3. Treatment with oleic acid reduces IgE binding to peanut and cashew allergens. Food Chem. (2015) 180: 295-300. Chung S.Y., et al.
  4. In vivo and T cell cross-reactivity between walnut, cashew and peanut. Int Arch AllergyImmunol. (2009) 148 (2): 109-17. Kulis M., et al.
  5. Decreased immunoglobulin E (IgE) binding to cashew allergens following sodium sulfite treatment and heating. J Agric Food Chem. (2014) 62 (28): 6746-55. Mattison C.P., et al.

ADDITIONAL INFORMATION

  • Full Name: Cashew ELISA Kit
  • Sample Type: Food
  • Sensitivity: 0.2 ppm

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Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford