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Casein ELISA Kit

  • Created on the 4 October, 2017.

BACKGROUND

Casein belongs to a family of phosphoproteins (αS1, αS2, β, κ) that are making up to 80% of the protein found in cow’s milk and approx. 20-45% of proteins present in human milk. The work casein comes from the Latin word caseus (meaning cheese), this is due the fact it represents a major ingredient in the production of cheese. Casein is frequently used as a food additive, types of glue (adhesive for labelling plastic bottles), in artists paint and by the dentist an agent in the re-minerlaization of tooth enamel. Some of the symptoms that appear immediately for a person having an allergy to casein include swelling of the mouth, tongue, face or throat, nasal congestion causing runny nose, coughing, sneezing and wheezing, red/itchy skin, rashes or hives as well as itchy eyes. Other symptoms which can take weeks to develop include: increase in mucous production, abdominal cramps, diarrhoea and bloody or loose stool. The allergy can in some cases be severe enough to trigger anaphylaxis, therefore it is recommended to avoid if possible. This casein ELISA assay is a highly sensitive detection system which is designed for the identification and quantification of bovine casein residues present in cookies, bread crumbs, chocolate, sausage, soy products, wine  and orange juice.

INTENDED USE

Casein ELISA kit is a procedure intended for quantitative measurement of bovine casein in food (such as bread crumbs, chocolate, cookies, soy products, sausage, orange juice and wine). This assay has a minimum analytical sensitivity limit of 0.04 ppm.

SENSITIVITY

The minimum detection sensitivity level of casein using this casein ELISA kit was 0.04 ppm. The standard assay range for this kit is 0.2 – 6.0 ppm.

REFERENCES

  1. Development of a sensitive ELISA for the detection of casein-containing fining agents in red and white wines. J Agric Food Chem. (2014) 62 (28): 6803-12. Deckwart M., et al.
  2. A simple and fast detection method for bovine milk residues in foods: a 2-site monoclonal antibody immunochromatography assay. J Food Sci. (2013) 78 (3): M452-7. Xuli W., et al.
  3. Specific antibodies in oral immunotherapy for cow’s milk allergy: kinetics and prediction of clinical outcome. Int Arch AllergyImmunol. (2014) 164 (1): 32-9. Savilahti E.M., et al.
  4. Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein. Mol Nutr Food Res. (2010) 54 (9): 1273-84. Stanic D., et al.
  5. Allergenicity of alpha-caseins from cow, sheep, and goat. Allergy. (1997) 52 (3): 293-8. Spuergin P., et al.
  6. Commercial Milk Enzyme-Linked Immunosorbent Assay (ELISA) Kit Reactivities to Purified Milk Proteins and Milk-Derived Ingredients. J Food Sci. (2016) 81 (7): T1871-8. Ivens K.O., et al.

ADDITIONAL INFORMATION

  • Full Name: Casein ELISA Kit
  • Sample Type: Food
  • Sensitivity: 0.04 ppm

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Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford