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Cardiotropin-1 (CT-1) ELISA Kit

  • Created on the 16 March, 2017.

BACKGROUND

Cardiotrophin-1 (CT-1) is a cardiac hypertrophic factor of 21.5 kDa and is a member of the family of cytokines which includes interleukin-6, ciliary neurotrophic factor, interleukin-11, oncostatin M and leukemia inhibitory factor. Human CT-1 protein is made up of 201 amino acids and it shares approx. 80% amino acid identity with the 203-amino acid that is found in mouse CT-1 sequence. However, unlike the mouse protein, that human CT-1 contains 2 instead of 1 cys and also has no N-glycosylation site. Even though it lacks a signal sequence, the secreted CT-1 and mouse CT-1 still have the ability to induce cardiac myocyte hypertrophy in cell culture and also can bind to both mouse and human LIFR but not to OSMR.

CT-1 is primarily associated with many pathophysiology of heart diseases, for example congestive heart failure, myocardial infarction, valvular heart disease and hypertension. This protein can exert many cellular effects by interacting with leukemia inhibitory factor receptor beta (LIFR)/glycoprotein 130 (gp130) heterodimer. Furthermore, a 1.7-kb CT-1 transcript has also been identified at high concentrations in heart, skeletal muscle, ovary and prostate. Low levels are detected in the kidney, thymus, pancreas, small intestine and testis. Little or no expression is seen in the brain, peripheral blood leukocytes, spleen, colon and placenta. Strong expression was observed in fetal kidney and lung. Finally, CT-1 can activate phosphatidylinositol 3-kinase (PI-3 kinase) activity in cardiac myocytes and also enhance the DNA -binding activities of the transcription factor NF-κB.

INTENDED USE

Cardiotrophin-1 ELISA kit can be used for detecting in vitro quantitative levels of cardiotrophin-1 (CT-1) in human serum, plasma (EDTA, heparin) and cell culture supernatant. This assay has a minimum detection limit of < 10.0 pg/ml.

SENSITIVITY

The minimum detection sensitivity level of cardiotrophin-1 (CT-1) using this human cardiotrophin-1 ELISA kit was approximately 10.0 pg/ml. The dynamic assay range for this kit is 31.2 – 2,000 pg/ml.

REFERENCES

  1. Cardiotrophin-1 (CT-1): a novel hypertrophic and cardioprotective agent. Int J Exp Pathol. (1999) 80 (4): 189-96. Review. Latchman D.S.
  2. Cardiotrophin-1 in cardiovascular regulation. Adv Clin Chem. (2010) 52: 41-76. Review. Jougasaki M.
  3. Vascular effects of glycoprotein130 ligands–part II: biomarkers and therapeutic targets. Vascul Pharmacol. (2012) 57 (1): 29-40. Review. Demyanets S., et al.
  4. Emerging evidence for the role of cardiotrophin-1 in cardiac repair in the infarcted heart. Cardiovasc Res. (2005) 65 (4): 782-92. Review. Freed D.H., et al.
  5. The Role of Leukemia Inhibitory Factor Receptor Signaling in Skeletal Muscle Growth, Injury and Disease. Adv Exp Med Biol. (2016) 900: 45-59. Review. Hunt L.C. and White J.
  6. Cardiotrophin-1: A multifaceted cytokine. Cytokine Growth Factor Rev. (2015) 26 (5):523-32. Review. López-Yoldi M., et al.

ADDITIONAL INFORMATION

  • Full Name: Cardiotrophin-1 (CT-1) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma (Heparin, EDTA), Cell Culture Supernatant, Serum
  • Sensitivity: < 10.0 pg/ml

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