GET A QUOTE

Cardiac Troponin I (cTn-I) ELISA Kit

  • Created on the 16 May, 2017.

BACKGROUND

Troponin I is an important cardiac and skeletal muscle protein and has been a vital tool for diagnosing heart attack. It belongs to the troponin protein complex and has the ability to bind actin in the thin myofilaments in order to hold the actin-tropomyosin complex together. Cardiac troponin I, which is often referred to as cTn-I, consists of 209 amino acids with a molecular weight of 23.9 kDa and it is present within cardiac muscle tissue as a single isoform. cTnI is found to differ from other troponins family members because of its 26 amino acids extension in the N-terminal. Multiple reaction which are able to monitor human cTnI has identified at least 14 phosphorylation sites and also found that the phosphorylation pattern in these sites can changed in response to disease. Troponin I, however, is not only specific for myocardial damage secondary to infarction but has been known to be elevated in heart failure, chronic renal failure, chronic renal failure and subarachnoid haemorrhage.

INTENDED USE

Human cardiac troponin I ELISA kit is a protocol for measuring in vitro quantitative concentrations of cardiac troponin I (cTn-I) in human cell culture supernatant, plasma, serum, cell lysates and other biological fluids. This assay has a minimum sensitivity limit of 2.54 ng/L.

SENSITIVITY

The minimum detection sensitivity level of human cTn-I using this human cardiac troponin I ELISA kit was 2.54 ng/L. The dynamic assay range for this kit is 5.0 – 1,500.0 ng/L.

REFERENCES

  1. Standardisation of cardiac troponin I measurement: past and present. Pathology. (2010) 42 (5): 402-8. Review. Tate J.R., et al.
  2. Cardiac troponin assays in the management of heart failure. Clin Chim Acta. (2015) 441: 92-8. Review. Torre M. and Jarolim P.
  3. Human cardiac troponin complex. Structure and functions. Biochemistry (Mosc). (2013) 78 (13): 1447-65. Review. Katrukha I.A.
  4. Diagnostics on acute myocardial infarction: Cardiac troponin biomarkers. Biosens Bioelectron. (2015) 70: 209-20. Review. Fathil M.F., et al.
  5. Integration of troponin I phosphorylation with cardiac regulatory networks. Circ Res. (2013) 112 (2): 355-66. Review. Solaro R.J., et al.
  6. Cardiac troponins in renal insufficiency: review and clinical implications. J Am Coll Cardiol. (2002) 40 (12): 2065-71. Review. Freda B.J., et al.

ADDITIONAL INFORMATION

  • Full Name: Cardiac Troponin I (cTn-I) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Other Biological Fluids, Plasma, Cell Culture Supernatant, Cell Lysates
  • Sensitivity: 2.54 ng/L

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford