Cardiac Troponin I ELISA Kit (cTn-I)

INTENDED USE

Human cardiac troponin I ELISA kit is a protocol for measuring in vitro quantitative concentrations of cardiac troponin I (cTn-I) in human cell culture supernatant, plasma, serum, cell lysates and other biological fluids. This assay has a minimum sensitivity limit of 9.38 pg/ml.

BACKGROUND

Troponin I (cTn-I) is an important cardiac and skeletal muscle protein and has been a vital tool for diagnosing heart attack. It belongs to the troponin protein complex and has the ability to bind actin in the thin myofilaments in order to hold the actin-tropomyosin complex together. It consists of 209 amino acids with a molecular weight of 23.9 kDa and it is present within cardiac muscle tissue as a single isoform.

Troponin I is found to differ from other family members because of its 26 amino acids extension in the N-terminal. Multiple reaction which are able to monitor cTnI has identified at least 14 phosphorylation sites and also found that the phosphorylation pattern in these sites can changed in response to disease.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microwell Plate: cTn-I antibody pre-coated wells.
• Standards: Concentrations 15.63, 31.25, 62.5, 125, 250, 500, 1,000 pg/ml
• Sample/Standard Diluent Buffer.
• Biotin Conjugated Antibody (Dilution 1:100).
• Antibody Diluent Buffer.
• Conjugate Reagent (Dilution 1:100).
• Diluent Buffer.
• TMB Substrate Reagent.
• Stop Solution.
• Wash buffer (25x).
• Plate Sealer.

SENSITIVITY

The minimum detection sensitivity level of human cardiac troponin I (cTn-I) using this cTn-I ELISA kit was 9.38 pg/ml. The dynamic assay range for this kit is 15.6 – 1,000.0 pg/ml.

ASSAY CHARACTERISTICS

– Intra Assay Precision: < 10%
– Inter Assay Precision: < 10%

REFERENCES

1. Standardisation of cardiac troponin-I measurement: past and present. Pathology. (2010) 42 (5): 402-8. Review. Tate J.R., et al.
2. Human cardiac troponin complex. Structure and functions. Biochemistry (Mosc). (2013) 78 (13): 1447-65. Review. Katrukha I.A.
3. Integration of troponin I phosphorylation with cardiac regulatory networks. Circ Res. (2013) 112 (2): 355-66. Review. Solaro R.J., et al.
4. Cardiac troponins in renal insufficiency: review and clinical implications. J Am Coll Cardiol. (2002) 40 (12): 2065-71. Review. Freda B.J., et al.

ADDITIONAL INFORMATION

• Full Name: Cardiac Troponin I ELISA Kit (cTn-I)
• Reactivity: Human
• Sample Type: Serum, Other Biological Fluids, Plasma, Cell Culture Supernatant, Cell Lysates
• Sensitivity: 9.38 pg/ml

OTHER RELATED ELISA KITS

• hs-cTn ELISA Kit
• Troponin T ELISA Kit
• Cardiotrophin-1 ELISA Kit