Candida Albicans IgM ELISA Kit

Full Name: Candida Albicans IgM ELISA Kit
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: 1.11 U/ml

BACKGROUND

Candida albicans belong to the yeast species and are a single celled fungus that predominately live in the gastrointestinal tract. There can be many different factors which are responsible for keeping check the balance of these microbes present within the body, however, if these become affected then this could result in the fungus growing out of control and resulting in yeast infections (known as candidiasis).

However, when certain factors disrupt this delicate balance, it can lead to an overgrowth of Candida albicans and subsequently cause yeast infections, or candidiasis. Various factors can contribute to the disruption of this microbial equilibrium. These may include changes in hormone levels, weakened immune system function, prolonged antibiotic use, high sugar diets, stress and more. When these factors come into play and upset the natural harmony of microorganisms within our body, Candida albicans can thrive and cause discomfort.

Yeast infections caused by Candida overgrowth can manifest in different parts of the body such as the mouth (oral thrush), genital area (vaginal yeast infection), or skin folds (intertrigo). Symptoms may vary but commonly include itching, burning sensations, redness or irritation in affected areas. It is important to address these infections promptly by seeking medical advice for proper diagnosis and treatment options. Antifungal medications are often prescribed to combat Candida overgrowth effectively. Maintaining a healthy lifestyle through balanced nutrition, regular exercise routine along with stress management techniques helps support overall well-being including keeping microbial imbalances at bay.

However, for majority of cases it does not cause as issues but they is the possibility of infections and overgrowth to occur. It has been reported that a mortality rate of approx. 40% to occur in individuals that display systemic candidiasis which is as a result of C.albicans.

INTENDED USE

Human Candida albicans IgM ELISA kit is designed for measuring in-vitro amounts of human IgM antibodies against Candida albicans (Candida IgM) in samples of plasma and serum. This assay has a minimum analytical sensitivity limit of 1.11 U/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Candida albicans antigen coated microtiter strips.
Enzyme Conjugate.
Calibrator B (Cut-Off Standard).
Calibrator D (Positive Control).
Calibrator C (Weak Positive Control).
Calibrator A (Negative Control).
TMB Substrate.
Sample Diluent.
Washing Buffer (10x).
Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of human IgM antibodies to Candida albicans (Candida-IgM) using this Candida IgM ELISA kit was 1.11 U/ml. The dynamic range for this assay is 1.0 – 150.0 U/ml.

ASSAY CHARACTERSTICS

– Intra-Assay-Precision: 9.4 %
– Inter-Assay-Precision: 8.6 %
– Inter-Lot-Precision: 4.9 – 11.7 %
– Analytical Sensitivity: 1.07 U/mL
– Recovery: 90 – 101 %
– Linearity: 88 – 128 %
– Cross-Reactivity: No cross-reactivity to Aspergillus, Bordetella und Helicobacter
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL
– Clinical Specificity: 98 %
– Clinical Sensitivity: 100 %

REFERENCES

Dendritic cells from X-linked hyper-IgM patients present impaired responses to Candida albicans and Paracoccidioides brasiliensis. J Allergy Clin Immunol. (2012) 129 (3): 778-86. Cabral-Marques O., et al.
In situ IgM production and clonal expansion of B-1 cells in peritoneal cavity promote elimination of C. albicans infection in IgH transgenic mice with VH derived from a natural antibody. PLoS One. (2013) 8 (4): e60779. Fu M., et al.
[Anti-Candida albicans IgM antibody: correlation between immunofluorescence and ultra centrifugation (author’s transl)]. Ann Biol Clin (Paris). (1978) 36 (6): 519-21. French. Guinet R. and Absi N.

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