Candida Albicans IgG ELISA Kit

Full Name: Candida Albicans IgG ELISA Kit
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: 1.11 U/ml

BACKGROUND

Candida albicans is a polymorphic fungus, meaning it can grow in different morphological forms including oval yeast cells, pseudohyphae, and true hyphae. The oval yeast cells reproduce asexually by budding off daughter cells. Pseudohyphae form by elongation of yeast cells into tube-like structures attached end-to-end. True hyphae are long filamentous structures with parallel-sided walls.

C. albicans transitions between these forms in response to environmental conditions and cues. Switching to the hyphal form is associated with increased virulence and tissue invasion. Biofilms of C. albicans on medical devices like catheters can serve as reservoirs for infection. C. albicans has factors like adhesins and invasins that facilitate attachment and penetration of host tissues. Its cell wall is composed of chitin, beta-glucans, and mannoproteins. Secreted aspartyl proteases and phospholipases allow degradation of host molecules.

INTENDED USE

Human Candida albicans IgG ELISA kit is a method for measuring in-vitro amounts of human IgG antibodies to Candida albicans (Candida IgG) using serum and plasma samples. This assay has a minimum analytical sensitivity limit of 1.11 U/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Candida albicans antigen coated microtiter strips.
Calibrator D (Positive Control).
Calibrator C (Weak Positive Control).
Calibrator B (Cut-Off Standard).
Calibrator A (Negative Control).
Sample Diluent.
Enzyme Conjugate.
TMB Substrate.
Washing Buffer (10x).
Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of human IgG antibodies against Candida albicans (Candida-IgG) using current Candida IgG ELISA kit was 1.11 U/ml. The dynamic range for this assay is 1.0 – 150.0 U/ml.

ASSAY CHARACTERSTICS

– Intra-Assay-Precision: 5.9 %
– Inter-Assay-Precision: 8.9 %
– Inter-Lot-Precision: 6.1 – 12.4 %
– Analytical Sensitivity: 1.11 U/mL
– Recovery: 93 – 111 %
– Linearity: 75 – 120 %
– Cross-Reactivity: No cross-reactivity to Aspergillus, Bordetella und Helicobacter
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL
– Clinical Specificity: 91 %
– Clinical Sensitivity: 94 %

REFERENCES

Detection of antibodies to Candida albicans germ tube in the diagnosis of systemic candidiasis. Eur J Clin Microbiol. (1987) 6 (2): 142-6. Quindós G., et al.
IgA and IgG serum antibodies to Candida-albicans in women of child-bearing age. Sabouraudia. (1983) 21 (3): 223-31. Schønheyder H., et al.
Salivary IgA and serum IgA and IgG antibodies to Candida albicans in HIV-infected subjects. Int J STD AIDS. (2002) 13 (6): 373-7. Belazi M., et al.
Enzyme-linked immunosorbent assay (ELISA) for IgM, IgG and IgA class antibodies against Candida albicans antigens: development and comparison with other methods. Sabouraudia. (1981) 19 (2): 123-34. Kostiala A.A. and Kostiala I.

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